Figure 1.

Representative schema for administration of treatment agents to mice. Mice were administered ID8-RFP tumor cells at day 0. Decitabine (1.25 mg/kg each dose or control PBS) and/or selinexor (10 mg/kg or control vehicle) was administered as single or in combination treatment at time points as indicated. Selinexor was reconstituted in vehicle consisting of 0.6% Plasdone PVP K-29/32 and 0.6% Poloxamer Pluronic F-68 in sterile distilled water. Panel (A) describes the basic regimen for treatment of mice. Panels (B–D) show the morphology of mice at the time of euthanasia. Mice were administered tumor cells followed by combination treatments. In this study, mice were euthanized on day 66 post-tumor cell inoculation. Panels show contrasting sizes for the abdomens in different groups. Panel (B) shows a normal unmanipulated mouse. Representative mice for treatment outcome with combination decitabine and selinexor (Panel (C), flat abdomens) and for PBS and vehicle controls (panel (D), enlarged abdomens) are shown. Results are representative of observations of two separate experiments. Enlarged abdomens were also found in single control PBS or control vehicle mice.