Figure 2.

CB-ECFC NOX4 activity and signalling was reduced by hypoxia. CB-ECFCs were cultured under hypoxia or normoxia for 48 h prior to the analysis of (A–C) mRNA expression of NOX4, NOS3 and HMOX-1 and (D–G) protein expression of NOX4, NOX2, eNOS and HO-1 using qRT-PCR and Western blot, respectively, with normalisation to ACTB/β-actin as reference control. Representative blots are shown from a single clone for each group. For scatter plots, data were mean ± SEM; n = 9 combined from three CB-ECFC clones; *** p < 0.001, **** p < 0.0001 versus normoxia, unpaired Student’s t-test.