Skip to main content
. 2023 Sep 6;12(18):2220. doi: 10.3390/cells12182220

Figure 7.

Figure 7

PLAC8 knockdown promoted increased phosphorylation of VEGFR2, STAT3 and c-Jun as downstream mediators of CB-ECFC angiogenic signalling in hypoxia. CB-ECFCs were transfected using either PLAC8-targeting siRNA or non-targeting SCR control for 24 h prior to culture in hypoxia or normoxia for 48 h and protein extraction. (A) A heatmap of relative changes in protein kinase phosphorylation using a human phospho-kinase proteome profiler array. The green and red colouring represent increased and decreased expressions, respectively. (B) Original blots incubated with 300µg of pooled protein lysate of triplicates from three CB-ECFC clones (1: c-Jun; 2: p53; 3: STAT3). (C–E) Protein expression of p-VEGFR2, p-STAT3 and p-c-Jun using Western blot with normalisation to β-actin as reference control. For scatter plots, data were mean ± SEM; n = 9 combined from three CB-ECFC clones; * p < 0.05 versus SCR control, unpaired Student’s t-test.