Figure 5.

Nano liquid chromatography parallel reaction monitoring/high-resolution mass spectrometry analysis of Cys-containing peptides from cisplatin-treated A549 media. Chromatograms show corresponding light (black) and heavy (red) transitions. Carbamidomethyl (CAM)-modified transitions indicate free (reduced) Cys proteoforms and N-ethyl maleimide (NEM)-modified transitions indicate disulfide (oxidized) Cys proteoforms. (A) tryptic peptide M(O)SSYAFFQTC²³R with CAM-modified transitions (left) and NEM-modified transitions (right). (B) Glu-C peptide FSK(Ac*)K(Ac*)C⁴⁵SE with CAM-modified transitions (left) and NEM-modified transitions (right). (C) tryptic peptide RPPSAFFLFC¹⁰⁶SEYRPK with CAM-modified transitions (left) and NEM-modified transitions (right).