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. 2023 Sep 5;13(9):1347. doi: 10.3390/biom13091347

Table 2.

Primers for genotyping dlx mutants.

Target Forward Sequence (5′-3′) Reverse Sequence Product Size
dlx1a WT CCTATGCGTCTCGGTCCATT CGTAGTGCGTCGACAAAAGC 740 bp
dlx1a mutant GTGTTTCTTCTCCGGTGCGA 320–350 bp
dlx2a WT GCATGAAACACATGGAAACCGA AGTCTCCACCTCACCCCTCA 629 bp
dlx2a mutant TTGCTGACGCGACATTGC CAAGTCCCAGGAATCGCCG 130–160 bp
dlx5a WT ATCAAGAACCAGGCGCATCT GAGCCCACACAGAGATAGCA 498 bp
dlx5a mutant CCCTTTTCAGCTCTCCACGAT 290–310 bp
dlx6a WT GGAGGCTCAAGACTCGTCAA TTGCTGACATACGACGGGG 429 bp
dlx6a mutant AAAACGATTCGCTTCCTGTC 200–250 bp
inter56 WT AGCTACATGCCCGGCTATTC GGACAAGATGCGGCTCTATTC 796 bp
inter56 mutant ATAGCCCCCAACCTACAAGC 350–400 bp
dlx5i6 WT GGAGGCTCAAGACTCGTCAA TTGCTGACATACGACGGGG 429 bp
dlx5i6 mutant CCCTTTTCAGCTCTCCACGAT 200–230 bp