Table 2.
Target | Forward Sequence (5′-3′) | Reverse Sequence | Product Size |
---|---|---|---|
dlx1a WT | CCTATGCGTCTCGGTCCATT | CGTAGTGCGTCGACAAAAGC | 740 bp |
dlx1a mutant | GTGTTTCTTCTCCGGTGCGA | 320–350 bp | |
dlx2a WT | GCATGAAACACATGGAAACCGA | AGTCTCCACCTCACCCCTCA | 629 bp |
dlx2a mutant | TTGCTGACGCGACATTGC | CAAGTCCCAGGAATCGCCG | 130–160 bp |
dlx5a WT | ATCAAGAACCAGGCGCATCT | GAGCCCACACAGAGATAGCA | 498 bp |
dlx5a mutant | CCCTTTTCAGCTCTCCACGAT | 290–310 bp | |
dlx6a WT | GGAGGCTCAAGACTCGTCAA | TTGCTGACATACGACGGGG | 429 bp |
dlx6a mutant | AAAACGATTCGCTTCCTGTC | 200–250 bp | |
inter56 WT | AGCTACATGCCCGGCTATTC | GGACAAGATGCGGCTCTATTC | 796 bp |
inter56 mutant | ATAGCCCCCAACCTACAAGC | 350–400 bp | |
dlx5i6 WT | GGAGGCTCAAGACTCGTCAA | TTGCTGACATACGACGGGG | 429 bp |
dlx5i6 mutant | CCCTTTTCAGCTCTCCACGAT | 200–230 bp |