Fig. 5.

Molar mass as a function of oligomeric size and SDS:protein ratio in the complex. Diagonal lines: The stoichiometry (black: 0.5:1, red: 0.75:1, green: 1:1 and blue: 1.25:1) has very little influence on the molar mass. Calculated molar masses based on the hydrodynamic measurements of the complex as a function of partial specific volume and stoichiometry (black: 0.7265 ml/g, red: 0.7272 ml/g, green: 0.7280 ml/g, blue: 0.7287 ml/g). These lines intersect very close to the hexameric configuration, proving that the complex is hexameric