KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Arg1 (M-20) | Santa Cruz Biotechnology | Cat#sc-18355; RRID: AB_2058957 |
| Anti-mouse CD68 (FA-11) | Bio-Rad | Cat# MCA1957GA; RRID: AB_324217 |
| Anti-CD8a (53–6.7) | Thermo Fisher Scientific | Cat# 14-0081-82; RRID: AB_467087 |
| Anti-mouse MMR/ CD206 | R and D Systems | Cat# AF2535; RRID: AB_2063012 |
| Anti-mouse CD16/32 (2.4G2) | BD Biosciences | Cat# 553142; RRID: AB_394657 |
| Anti-FOXP3 (FJK-16s) | Thermo Fisher Scientific | Cat# 14-5773-82; RRID: AB_467576 |
| Anti-GLUT1 (SA0377) | Thermo Fisher Scientific | Cat# MA5-31960; RRID: AB_2809254 |
| Anti-Iba1 | FUJIFILM Wako Shibayagi | Cat# 019-19741; RRID: AB_839504 |
| Anti-mouse CD49d / integrin alpha4 (9C10 (MFR4.B)) | BioLegend | Cat# 103701; RRID: AB_313042 |
| Anti-Ki67 | Abcam | Cat# ab15580; RRID: AB_443209 |
| Anti-cMyc tag | Novus | Cat# NB600-335; RRID: AB_10002720 |
| Anti-CD31/ PECAM1 | Abcam | Cat# ab28364; RRID: AB_726362 |
| Anti-PU.1 (E.388.3) | Thermo Fisher Scientific | Cat# MA5-15064; RRID: AB_10986949 |
| Anti-Pimonidazole Dylight 594 (4.3.11.3) | Hydroxyprobe | Cat# HP7-100Kit |
| Bacterial and virus strains | ||
| Stbl3 E. coli | Thermo Fisher Scientific | C737303 |
| Biological samples | ||
| Primary bone marrow derived macrophages (mouse) | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Pimonidazole | Hydroxyprobe | Cat# HP-100mg |
| Evofosfamide/ TH-302 | AdooQ Bioscience | Cat# A11144 |
| Recombinant murine M-CSF | Peprotech | Cat# 315-02 |
| Percoll | GE Healthcare | Cat# 17089101 |
| BrainBits Hibernate E Low Fluorescence | Fisher Scientific | Cat# NC0285514 |
| DNAse I | Worthington | Cat# LS006344 |
| RBC lysis buffer | BioLegend | Cat# 420301 |
| Critical commercial assays | ||
| Mouse IL-1 beta/IL-1F2 DuoSet ELISA | R and D Systems | Cat# DY401 |
| DuoSet ELISA Ancillary Reagent Kit 2 | R and D Systems | Cat# DY008 |
| RNeasy mini kit | Qiagen | Cat# 74104 |
| SuperScript III First Strand Synthesis System | Thermo Fisher Scientific | Cat# 18080051 |
| Neural Tissue Dissociation Kit (P) | Miltenyi Biotec | Cat# 130-092-628 |
| Deposited data | ||
| Analyzed scRNA-seq data | This paper | Gene Expression Omnibus, GEO: GSE179077 |
| Experimental models: Cell lines | ||
| GL261 (murine GBM cell line) | National Cancer Institute | NCI-DTP Cat# Glioma 261; RRID: CVCL_Y003 |
| GL261-HRE-UnaG | This paper | N/A |
| CT2A | Millipore | Millipore Cat# SCC194, RRID: CVCL_ZJ44 |
| HEK293T | ATCC | CRL-3216; RRID: CVCL_0063 |
| Experimental models: Organisms/strains | ||
| C57BL/6J | The Jackson Laboratory | Strain# 000664; RRID: IMSR_JAX:000664 |
| C57BL/6J SCID; B6.Cg-Prkdcscid/SzJ | The Jackson Laboratory | Strain# 001913; RRID: IMSR_JAX:001913 |
| ICR-SCID; IcrTac:ICR-Prkdcscid | Taconic Biosciences | Model# ICRSC-M; RRID: IMSR_TAC:icrsc |
| N-tva-Ccl8/12 KO | Sabina Islam | N/A |
| N-tva-Il1b KO | Dmitry Shayakhmetov | N/A |
| Oligonucleotides | ||
| Mouse Arg-1 forward: 5’-GTGGCTTTAACCTTGGCTTG-3’ | This paper | N/A |
| Mouse Arg-1 reverse: 5’-CTGTCTGCTTTGCTGTGATG-3’ | This paper | N/A |
| Mouse Ccl8 forward: 5’-ACAATATCCAGTGCCCCATG-3’ | This paper | N/A |
| Mouse Ccl8 reverse: 5’-TGAAGGTTCAAGGCTGCAG-3’ | This paper | N/A |
| Mouse CD68 forward: 5’-ACTTCGGGCCATGTTTCTCT-3’ | This paper | N/A |
| Mouse CD68 reverse: 5’-GCTGGTAGGTTGATTGTCGT-3’ | This paper | N/A |
| Mouse Il1b forward: 5’-CCAAGCAACGACAAAATACC-3’ | This paper | N/A |
| Mouse Il1b reverse: 5’-GTTGAAGACAAACCGTTTTTCC-3’ | This paper | N/A |
| Mouse Gapdh forward: 5’-ACTGCCACCCAGAAGACTGT-3’ | This paper | N/A |
| Mouse Gapdh reverse: 5’-GATGCAGGGATGATGTTCT-3’ | This paper | N/A |
| Recombinant DNA | ||
| pcDNA-dUnaG | Dr. Friedemann Kiefer | N/A |
| pENTR/D-TOPO | Thermo Fisher Scientific | Cat#: K240020 |
| pLenti X1 Puro DEST | Addgene | Plasmid# 17297; RRID: Addgene_17297 |
| pMD2.G | Addgene | Plasmid# 12259; RRID: Addgene_12259 |
| psPAX2 | Addgene | Plasmid# 12260; RRID: Addgene_12260 |
| pLenti-HRE-dUnaG | This paper | Addgene #124372 |
| Software and algorithms | ||
| Microsoft Office | Microsoft | RRID: SCR_016137 |
| Adobe Photoshop | Adobe | RRID: SCR_014199 |
| Fiji Is Just ImageJ (FIJI) | Public domain, National Institutes of Health | https://imagej.net/software/fiji/; RRID: SCR_003070 |
| Prism 9 | GraphPad | RRID: SCR_002798 |
| SnapGene | Dotmatics | RRID: SCR_015052 |
| Zen Blue | Zeiss | RRID: SCR_013672 |
| Cell Ranger | 10x Genomics | RRID: SCR_017344 |
| Seurat R package | Rahul Satija Lab | https://satijalab.org/seurat/index.html; RRID: SCR_007322 |
| SCRABBLE R package | Kai Tan Lab | https://github.com/tanlabcode/SCRABBLE |
| Enrichr | Avi Ma’ayan Lab | https://maayanlab.cloud/Enrichr/; RRID: SCR_001575 |
| GBM Bio Discovery Portal | NCI | https://gbm-biodp.nci.nih.gov |
| cBioPortal for Cancer Genomics | MSKCC | https://docs.cbioportal.org/about-us/; RRID: SCR_014555 |
| Ivy Glioblastoma Atlas Project | Allen Institute for Brain Science | https://glioblastoma.alleninstitute.org; RRID: SCR_005044 |
| high dimensional WGCNA R package | Vivek Swarup Lab | https://smorabit.github.io/hdWGCNA/index.html |
| Leica Application Suite X | Leica Microsystems | RRID: SCR_013673 |
| Ingenuity Pathway Analysis | Qiagen Digital Insights | RRID: SCR_008653 |
| Gene Set Enrichment Analysis | Broad Institute | RRID: SCR_003199 |
| The Cancer Genome Atlas | National Institutes of Health | https://www.cancer.gov/about-nci/organization/ccg/research/structural-genomics/tcga; RRID: SCR_003193 |
| Monocle3 | Cole Trapnell Lab | https://cole-trapnell-lab.github.io/monocle3/; RRID: SCR_018685 |
| CellPhoneDB 4.0 | Roser Vento-Tormo Lab | https://github.com/ventolab/CellphoneDB; RRID: SCR_017054 |
| GlioVis | Public domain, Massimo Squatrito Lab | http://gliovis.bioinfo.cnio.es |