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. 2023 Sep 11;12(18):2247. doi: 10.3390/cells12182247

Figure 4.

Figure 4

Obatoclax induces autophagy in P525L FUS e-GFP iPSC-derived neurons. (a) Obatoclax was tested at 10 nM for 6, 9, 24, and 48 hours. We assessed the LC3B-II protein levels using Western blotting. Results show the mean of six independent experiments (n = 6); error bars indicate SEM. Treatments were analyzed via one-way ANOVA with a Dunnett post-test, * indicates p < 0.5; n.s. indicates not significant. (b) Obatoclax was tested at 10 nM for 24 hours with and without Bafilomycin A at 10 nM for 24h. We assessed the LC3B-II protein levels using Western blotting. Results show the mean of three independent experiments (n = 3); error bars indicate SEM. Treatments were analyzed via one-way ANOVA with a Dunnet post-test; * indicates p < 0.05. ** indicates p < 0.01. n.s. indicates not significant. BafA1 = Bafilomycin A1. (c) Obatoclax was tested at 10 nM for 24 hours with and without Bafilomycin A at 10 nM for 48 hours. We assessed the LC3B-II protein levels using Western blotting. Results show the mean of five independent experiments (n = 5); error bars indicate SEM. Treatments were analyzed via one-way ANOVA with a Dunnet post-test, ** indicates p < 0.01. n.s., not significant. BafA1 = Bafilomycin A1. (d) Obatoclax was tested at 10 nM for 24 hours. We assessed the LAMP1 protein using Western blotting. Results show the mean of eight independent experiments (n = 8); error bars indicate SEM. n.s. indicates not significant. Related to Figures S7 and S8.