KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-Tau (Ser202, Thr205) Antibody (AT8) | Thermo fisher | MN1020, RRID:AB_223647 |
| Anti-α-Syn (phosphor-S129) Antibody | Abcam | ab59264, RRID:AB_2270761 |
| Anti-β-Amyloid, 1-16 Antibody (clone 6E10) | Biolegend | SIG-39300, RRID:AB_662807 |
| Ubiquitin Antibody | Santa Cruz | SC-8017, RRID:AB_628423 |
| Anti-Aggregated α-Synuclein Antibody (clone 5G4) | Millipore | MABN389, RRID:AB_2716647 |
| Anti-rabbit IgG(H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) | Cell signaling | 8889, RRID:AB_2716249 |
| Anti-mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate) | Cell signaling | 8890, RRID:AB_2714182 |
| Bacterial and virus strains | ||
| E. coli BL21 (DE3) | Tiangen Biotech | Cat# CB105 |
| AAV9-A53T-aSyn | Emory Virus Core | N/A |
| Biological Samples | ||
| Human brain tissue (see detail information in Table S6) | Emory ADRC | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Human α-Syn pre-formed fibrils | Homemade | N/A |
| Beta-amyloid (1-42) | ANA Spec | AS-GMP-20276-1 |
| Human recombinant Tau | Homemade | N/A |
| rotenone | ULTRA Scientific | Cat# PST-890 |
| ThT | Sigma | S96200 |
| ThS | Sigma | T1892 |
| Critical commertial assays | ||
| Histostain-SP kit | Invitrogen | Cat# 95-9943 |
| Deposited Data | ||
| α-Syn fibril-F0502B complex cryo-EM map | Electron Microscopy Data Bank #EMD-32615 | https://www.emdataresource.org/EMD-32615 |
| α-Syn fibril-F0502B complex atomic model | Protein Data Bank #7WMM | https://www.rcsb.org/structure/7WMM |
| apo-α-Syn fibrils structural models | Protein Data Bank #6A6B | https://www.rcsb.org/structure/6A6B |
| MSA type I structural models | Protein Data Bank #6XYO | https://www.rcsb.org/structure/6XYO |
| MSA type II1 structural models | Protein Data Bank #6XYP | https://www.rcsb.org/structure/6XYP |
| MSA type II2 structural models | Protein Data Bank #6XYQ | https://www.rcsb.org/structure/6XYQ |
| Lewy fold structural models | Protein Data Bank #8A9L | https://www.rcsb.org/structure/8A9L |
| Experimental models: Organisms/strains | ||
| 3XTG mice | The Jackson Laboratory | 034830, RRID:MMRRC_034830-JAX |
| 5xFAD mice | The Jackson Laboratory | 034840, RRID:MMRRC_034840-JAX |
| Tau P301S mice | The Jackson Laboratory | 008169, RRID:IMSR_JAX:008169 |
| SNCA Tg mice | The Jackson Laboratory | 017682, RRID:IMSR_JAX:017682 |
| Software and algorithms | ||
| Image J 1.53 | Schneider et al.60 | https://imagej.nih.gov/ij/ |
| Graph Pad Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism |
| FV31S-DT 2.6 | Olympus | https://www.olympus-lifescience.com/ |
| RELION 3.1 | Scheres32 | https://relion.readthedocs.io/en/release-3.1/index.html |
| COOT | Emsley et al.61 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PHENIX | Adams et al.62 | https://phenix-online.org/ |
| PMOD | Qiu et al.63 | https://www.pmod.com/web/ |
| syngo.via | Bindoli et al.64 | https://www.siemens-healthineers.cn/medical-imaging-it/clinical-imaging-applications |