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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Mol Cancer Res. 2023 Sep 1;21(9):975–990. doi: 10.1158/1541-7786.MCR-22-0715

Figure 2: OSMR is essential for OSM-mediated stem-like/mesenchymal conversion and is correlated with poor prognosis.

Figure 2:

A RNA expression of LIFR and OSMR from patient non-cancerous (N) versus cancerous (C) pancreatic tissues from the Oncomine data mining platform (Badea Pancreas dataset, from 39 normal and 39 PDAC patients; (68)). B Kaplan-Meier plot of patients with pancreatic cancer that express high or low levels of OSMR or IL6ST using the median as the cutoff value for high and low expression (33). Statistical significance was determined by KM-plotter where **P<0.01. C HPAC cells were infected with Lenti-CRISPRV2s targeting OSMR (SgOR1-SgOR4; or a control scrambled guide, SgS). Western blot of selected populations confirms SgOR1 and SgOR3 most efficiently knock-out OSMR (indicated by the asterisk*); the lower two bands are non-specific or “n.s.”. D-F Individual clones selected from the SgS, SgOR1, and SgOR3 populations were infected with lentiviruses encoding OSM (or a control lentivirus, VEC) and assessed by Western blot D, quantitative PCR E, or flow cytometry F, for the indicated genes and proteins. D Data are shown as mean ± S.E.M, and statistical significance was determined by two-way ANOVA where ****P<0.0001.