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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Mol Cancer Res. 2023 Sep 1;21(9):958–974. doi: 10.1158/1541-7786.MCR-23-0108

Figure 2.

Figure 2.

(A) IHC analysis of Integrin αv and Gal-3 expression in prostate tumor. Representative images of triple IHC staining of Integrin αv (red), Gal-3 (green), and E-cadherin (brown) in normal prostate and tumor tissues from PCa patients with different grades. (B) Representative images of triple IHC staining of Na+/K+-ATPase (red), Integrin αv (brown), and Gal-3 (green) in tumor tissues from PCa patients: non-metastatic (grade 4) and mCRPC patients with tissue or bone metastasis. Black boxes are highlighted at the right to present deconvoluted images of Gal-3 and Integrin αv. Bars for A and B, 100 μm. (C, D) Quantification of Integrin αv (C) and Gal-3 (D) H-scores at PM in patients from A; this includes only patients that demonstrate the correlation of parameters in different combinations of anti-Integrin αv and anti-Gal-3 Abs (see Supplemental Table S1 and supplemental methods). Pairwise comparisons using Wilcoxon rank sum exact test, p-adjusted using Benjamini-Hochberg. (E) Quantification of Integrin αv and Gal-3 colocalization at PM within different grades of PCa from images in A. Pairwise comparisons using Wilcoxon rank sum exact test, p-adjusted using Benjamini-Hochberg. (F, G) Quantification of Integrin αv (F) and Gal-3 (G) H-score at PM in patients from B. Pairwise comparisons using Wilcoxon rank sum exact test, p-adjusted using Benjamini-Hochberg. For G, non-metastatic group includes patients with all grades. (H) Quantification of Integrin αv and Gal-3 colocalization at PM in patients from B. Pairwise comparisons using Wilcoxon rank sum exact test, p-adjusted using Benjamini-Hochberg. (I) Representative images of triple IHC staining of Na+/K+-ATPase (red), Integrin αv (brown), and PHA-L lectin (green) in the tumor tissues from PCa patients: non-metastatic (grade 4) and CRPC patients with tissue or bone metastasis. Black boxes are presented at the right as deconvoluted images of Integrin αv and PHA-L lectin; bars, 100 μm. (J) Quantification of Integrin αv/PHA-L PM colocalization for samples from I; non-metastatic group includes patients with all grades Pairwise comparisons using Wilcoxon rank sum exact test, p-adjusted using Benjamini-Hochberg. For all graphs, n indicates the number of tissue samples. For all statistics: ****p<0.0001, ***p<0.001, **p<0.01, and *p<0.05, median ± SD.