Skip to main content
. Author manuscript; available in PMC: 2024 Mar 6.
Published in final edited form as: Cancer Discov. 2023 Sep 6;13(9):2072–2089. doi: 10.1158/2159-8290.CD-22-0874

Fig. 3: Tetramerization is necessary but not sufficient for transformation.

Fig. 3:

(A) Recombinant FH was analyzed by Native-PAGE with Coomassie staining. Enzyme separated out to tetramers, dimers, and monomers, indicated as T, D, and M respectively. (B) Densitometry was used to quantify the percentage of each multimerization species for each variant. (C) Percent tetramerization for all variants that did not fit Michaelis-Menten kinetics in Fig. 2. Wildtype (WT) percent tetramer is indicated as a dotted line. All variants that are within 1 standard deviation of WT percent tetramer are indicated by darker gray bars.