Figure 2: Quantification of H3K9Ac and H3K4Ac in preactivated-HSCs.

a,d) Box-jitter plot of H3K9Ac and H3K4Ac fluorescence intensity as a function of stiffness. Every data point represents an individual microarray island, where the color of each datapoint is a specific ECM combination. b,e) Fluorescence Images of HSCs microarray islands. Scale Bar 100 microns. c,f) Box plots for H3K9Ac and H3K4Ac fluorescence intensity as a function of specific ECM combination on 1kPa, 6kPa and 25kPa. Boxplots: ‘*’: p-value <0.05; ‘****’: p-value <0.0001, calculated using Wilcox test in R, n>= 3 biological replicates (independent experiments) and n>= 10 technical replicates (individual islands).