Figure 8: Quantification of H3K9Me and H3K9Ac as a function of HSD11b1 and CEBPb knockdown.

a) Heatmap of the H3K9Me fluorescence intensity quantified in HSCs on the combinatorial microarrays b,) Box Plot of quantification of Lox fluorescent intensity of HSCs on 1kPa, C4TC and 25kPa, FNLN respectively c) Representative images for the H3K9Me immunostain for NC and CEBPb KD HSCs on 1kPa, C4TC, DZnep. Scale Bar 100 microns e) Representative Image for the H3K9Me immunostain for NC and HSD KD HSCs on 25kPa, FNLN, DZnep. Scale Bar 100 microns h) Heatmap of the H3K9Ac fluorescence intensity quantified in HSCs on the combinatorial microarrays g,j) Box Plot of quantification of H3K9Ac fluorescent intensity of HSCs on 1kPa,C4TC and 25kPa, FNLN respectively f) Representative images for the H3K9Ac immunostain for NC and CEBPb KD HSCs on 1kPa, C4TC, DZnep. Scale Bar 100 microns i) Representative images for the H3K9Ac immunostain for NC and HSD KD HSCs on 25kPa, FNLN, DZnep. Scale Bar 100 microns