| Chemicals, peptides, and recombinant proteins |
| PBS |
Sigma |
Cat#P3813 |
| Succinimidyl acetate |
TCI |
Cat#S0878 |
| DMSO |
Sigma |
Cat#276855 |
| NH4HCO3
|
Sigma |
Cat#09830 |
| Peptide desalting spin column |
Pierce |
Cat#89851 |
| TFA |
Sigma |
Cat#302031 |
| Thioflavin T |
EMD Millipore |
Cat#596200 |
| Lipofectamine 2000 |
Invitrogen |
Cat#11668500 |
| Opti-MEM |
Gibco |
Cat#31985088 |
| Trypsin-EDTA |
Fischer Scientific |
Cat#25300120 |
| 2% paraformaldehyde |
EMS |
Cat#15710 |
| See Table S1 for sequences of tau peptides |
This study |
N/A |
| Deposited data |
| EM images and deposited map of the R1R2_23Ac fibril structure |
This study |
EMD-28721 |
| Coordinates of the R1R2_23Ac fibril structure |
This study |
8FNZ |
| Experimental models: Cell lines |
| Tau RD P301S HEK293T biosensors |
ATCC |
Cat#CRL-3275 |
| Software and algorithms |
| SerialEM |
Mastronarde, D. N. 200547
|
https://bio3d.colorado.edu/SerialEM
|
| CTFFIND v4.1 |
Rohou, A. & Grigorieff, N. 201548
|
https://grigoriefflab.umassmed.edu/ctffind4
|
| RELION v3.1 |
Scheres, S. H. W. 202036
|
https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
|
| EMAN v2.31 |
Tang, G. et al. 200749
|
https://blake.bcm.edu/emanwiki/EMAN2/Install/BinaryInstallAnaconda/2.31
|
| crYOLO v1.8.0 |
Wagner, T. et al. 202050
|
https://github.com/MPI-Dortmund/cryolo/blob/7b138d25f573be097662b0e0fc696b56c5486113/source/installation.rst] |
| COOT v9.4 |
Emsley, P. et al. 201053
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| ddG fibril analysis |
Mullapudi, V. et al. 202338
|
https://git.biohpc.swmed.edu/s184069/flex_ddg_ala_scn_runner
|
| Rosetta v3.12 |
Leman, J. K. et al. 202058
|
https://www.rosettacommons.org/
|
| FlowJo v10 |
BD |
https://www.flowjo.com/solutions/flowjo
|
| GraphPad Prism 9.4.1 |
Treestar |
https://www.graphpad.com/
|
| Other |
| Formvar-coated 300-mesh copper grids |
EMS |
Cat# FCF200CU-50 |
| R1.2/1.3, 300 mesh copper grids |
EMS |
Cat# Q350CR1.3 |
