Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-neurofilament heavy | Abcam | RRID:AB_304560; Cat.# ab4680 |
| anti-Calbindin D-28k | Swant | RRID:AB_100003; Cat.# CB-38a |
| anti-CGRP | Abcam | RRID:AB_114157; Cat.# ab47027 |
| anti-S100b | Abcam | RRID:AB_882426; Cat.# ab52642 |
| anti-beta III tubulin | Abcam | RRID:AB_444319; Cat.# AB_18207 |
| anti-TH | Abcam | RRID:AB_152453; Cat.# ab76442 |
| anti-TRKA | Biotechne R&D System | RRID:AB_228304; Cat.#AF1056-SP |
| anti-CGRP | Abcam | RRID:AB_165841; Cat.# ab81887 |
| AlexaFluor 647 | Invitrogen | RRID:AB_253586; Cat.# A21449 |
| AlexaFluor 594 | Invitrogen | RRID:AB_141637; Cat.# A21207 |
| Isolectin GS-IB4 from Griffonia simplicifolia, Alexa FluorTM 647 Conjugate | Thermo Fisher | RRID:SCR_014365; Cat.# I32450 |
| Lycopersicon Esculentum (Tomato) Lectin, DyLight 649 Invitrogen | Invitrogen | Cat.# L32472 |
| Chemicals, peptides, and recombinant proteins | ||
| FM 1–43 | Invitrogen | Cat.# T3163 |
| FM 1–43FX | Invitrogen | Cat.# F35355 |
| FM 4–64FX | Invitrogen | Cat.# F34653 |
| Capsaicin | Sigma | Cat.# M2028 |
| FastBlue tracer | Polysciences | Cat.# 17740–1 |
| Ruthenium Red | Sigma | Cat.# R2751 |
| ADVASEP-7 | Biotium | Cat.# 70029 |
| O.C.T. | Fisher Healthcare | Cat.# 23-730-571 |
| Tissue Tek O.C.T. | Sakura | Cat.# 4583 |
| Gold anti-fade mounting medium | Thermo Fisher | Cat.# S36939 |
| Fluoromount G mounting medium with DAPI | Thermo Fisher | Cat.# 00-4959-52 |
| Superfrost Plus microscope slides | Fisher | Cat.# 12-550-15 |
| Lipofectamine 2000 | Thermo Fisher | Cat.# 11668019 |
| PEI MAX | Polysciences | Cat.# 24765 |
| Dmem, high glucose, pyruvate | Thermo Fisher | Cat.# 11995073 |
| Penicillin-Streptomycin (10,000 U/mL) | Thermo Fisher | Cat.# 15140122 |
| Matrigel | Corning | Cat.# 354234 |
| MEM (Minimum Essential Medium) | Thermo Fisher | Cat.# 11095080 |
| Gibco Normal Goat Serum | Thermo Fisher | Cat.# PCN5000 |
| Donkey Serum | Sigma | Cat.# D9663 |
| Experimental models: Cell lines | ||
| Neuro2A-P1ko | Gary Lewin56 | N/A |
| Piezo1 knock-out HEK293T (HEK P1KO) | ATCC | Cat.# CRL-3519 |
| Experimental models: Organisms/strains | ||
| C57BL/6J | Jackson Laboratories | RRID:IMSR_JAX:000664 |
| CGRPcre+;GFP | Mark J. Zylka35 | N/A |
| HoxB8Cre+;Piezo2f/f | Previously reported in 4 | N/A |
| Phox2bCre; Piezo2f/f | Previously reported in 6 | N/A |
| Recombinant DNA | ||
| hPIEZO2- codon-optimized-T2A-BFP-WPRE (“PIEZO2”) | This paper, Table S1 | N/A |
| hPIEZO2-codon-optimized_mCherry-WPRE | This paper, Table S2 | N/A |
| hPIEZO2_R2686H-codon-optimized-T2A-BFP-WPRE (“PIEZO2R2686H) | This paper, Table S3 | N/A |
| hPiezo1-pIRES2-EGFP | Previously reported in 57 | N/A |
| hPiezo1-pIRES2-tdTomato | This paper, Table S4 | N/A |
| Rat TRPV1 | Previously reported in 58 | N/A |
| Software and algorithms | ||
| FIJI (ImageJ) | Schindelin et. al. (2012) doi: 10.1038/nmeth.2019 | RRID:SCR_002285 |
| Imaris (version 9.9.1) | Oxford Instruments | RRID:SCR_007370 |
| Prism (GraphPad 9.2.0) | GraphPad Software | RRID:SCR_002798 |
| Microsoft Excel | Microsoft | RRID:SCR_016137 |
| FlowJo 10.7.2 | BD | RRID:SCR_008520 |
| Igor Pro 8.02 | Wavemetrics | RRID:SCR_000325 |
| pClamp software (version 10.7) | Molecular Devices | RRID:SCR_011323 |
| Patchmaster | HEKA Elektronik | RRID:SCR_000034 |
| Other | ||
| EPC10 amplifier | HEKA Elektronik | N/A |
| HSPC-1 | ALA Scientific Instruments | N/A |
| MultiClamp700A amplifier | Molecular Devices | N/A |
| DigiData1550 | Molecular Devices | N/A |