Role of Pi16 deletion in inflammatory pain. (A, D)
Inflammatory pain was induced by intraplantar CFA injection. Mechanical
allodynia was assessed at baseline and over time using von Frey filaments in
female (A) and male (D) mice. Mixed-effects analysis,
Šidák’s multiple comparisons test: WT CFA vs
Pi16−/− CFA: *P<0.05,
**P<0.01, ***P<0.005, ****P<0.0001, WT PBS vs WT CFA:
#P<0.05, ###P<0.01, ####P<0.0001. (B, E) Thermal
sensitivity was assessed at baseline and over time in female (B) and male (E)
mice using Hargreaves’ test. Mixed-effects analysis,
Šidák’s multiple comparisons test: *P<0.05,
**P<0.01, ***P<0.005, ****P<0.0001. (C, F) Hind paw
thickness at baseline and over time following intraplantar CFA injection in
female (C) and male (F) mice was measured using a Digimatic micrometer. Two-way
ANOVA, Šidák’s multiple comparisons test.