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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Brain Behav Immun. 2023 Jun 12;112:220–234. doi: 10.1016/j.bbi.2023.06.011

Fig. 12.

Fig. 12.

PI16 deletion is associated with reduced macrophage infiltration of hindpaw skin following CFA. (A) qPCR shows no significant expression changes in Pi16 in WT skin, or Itgam/CD11b in female Pi16−/− versus WT hindpaw skin. Significant increases in expression of T-cell markers CD4 and CD8 occur only at late-stage timepoints. (B) Hindpaw skin shows significantly reduced CD68 (magenta) immunoreactivity along with an increase in the area fraction of CD206hi (yellow) cells in female Pi16−/− mice. DAPI: blue. Scale bar: 100 μm. (D) Representative IHC in vehicle (VEH) and tamoxifen-induced fibroblast PI16 knockout (Col1a2-cre) plantar hindpaw skin, 3 days after intraplantar CFA. (E) Significant decrease in CD68+ cell density in skin from mice with fibroblast-specific deletion of PI16. Significant increase in CD206hi cell density in skin from mice with fibroblast-specific deletion of PI16. Student’s T-test: *P<0.05, **P<0.01, Student’s T-test.