Fig. 6.

(A) Representative confocal images of DRG from PBS and CFA mice 3 days post-injection showing immunostaining of CD45 (green). DAPI (blue). Scale bar: 50 μm. CD45 density quantified in (B). (C) qPCR of Cd11b in WT and Pi16^−/− DRG 3d after PBS or CFA injection shows no significant differences in transcript level. (D) Flow cytometry of lumbar DRG shows elevated CD45⁺ cell numbers selectively in female Pi16^−/− at 8d post-CFA injection. (E) qPCR shows no significant differences between WT and Pi16^−/− inflammatory marker expression in DRG after intraplantar CFA injection.