Skip to main content
. Author manuscript; available in PMC: 2024 Sep 15.
Published in final edited form as: J Neuroimmunol. 2023 Aug 2;382:578168. doi: 10.1016/j.jneuroim.2023.578168

Fig 1. Neurons have prolonged STAT1 response to pathological IFN-γ compared to physiological IFN-γ.

Fig 1.

(A) Primary mature neurons were treated on (B) DIV5 or (C) DIV12 with physiological (gray; 0.02 U/mL) or pathological levels (cyan; 100 U/mL) of IFN-γ for 30 minutes then washed out; pSTAT1 and STAT1 were measured by western blot. (D) Primary mature neurons were treated with physiological (gray; 0.02 U/mL) or pathological levels (cyan; 100 U/mL) of IFN-γ for 30 minutes on (E) DIV5 or (F) DIV12, then re-stimulated 48 hours later with physiological or pathological IFN-γ. pSTAT1 levels were measured by western blot. Male and female mice included. Dotted lines represent SEM. (B) Two-Way ANOVA, repeated measures: pSTAT1: main effect of time *p<0.05 and dose **p<0.005, N=2–5; STAT1: main effect of time ***p<0.0005 and dose ***p<0.0005, interaction ***p<0.0005; N=2–5). (C) Mixed Effects Analysis, repeated measures: pSTAT1: main effect of time ***p<0.0005 and dose ***p<0.0005, interaction *p<0.05, N=6; STAT1: main effect of time *p<0.05, dose p=0.06, interaction **p<0.005; N=6). (E) Two Way ANOVA: main effect of dose ***p<0.0005 and restim *p<0.05, interaction *p<0.05; post-hoc Sidak’s multiple comparison: 100U/ml **p<0.005; N=3–5. (F) Two Way ANOVA: main effect of dose ***p<0.0005 and restim ***p<0.0005, interaction ***p<0.0005; post-hoc Sidak’s multiple comparison: 100U/ml ***p<0.0005; N=2–4.