| Antibodies |
| BRD4 (for WB) |
Cell Signaling Technology |
Cat#13440 |
| β-Actin (for WB) |
Cell Signaling Technology |
Cat#3700 |
| V5-Tag (for WB) |
Cell Signaling Technology |
Cat#13202 |
| HA-Tag (for WB) |
Cell Signaling Technology |
Cat#3724S |
| CDK9 (for WB) |
Cell Signaling Technology |
Cat#2316 |
| Cyclin T1 (for WB and ChIP) |
Cell Signaling Technology |
Cat#81464 |
| Tubulin (for WB) |
Developmental Studies Hybridoma Bank |
Cat#E7 |
| FLAG (for WB) |
Millipore Sigma |
Cat#F1804 |
| GFP (for WB) |
Santa Cruz Biotechnology |
Cat#sc-9996 |
| H3 (for WB) |
This study |
NA |
| BRDT (for WB) |
This study |
NA |
| Rpb1 (for ChIP) |
Cell Signaling Technology |
Cat#D8L4Y |
| Ser2p (for ChIP) |
Active Motif |
Cat#61984 |
| CDK9 (for ChIP) |
Santa Cruz Biotechnology |
Cat#sc-13130 X |
| GFP (for ChIP) |
Developmental Studies Hybridoma Bank |
Cat#GFP-G1 |
| GFP (for ChIP) |
Developmental Studies Hybridoma Bank |
Cat#GFP-12A6 |
| GFP (for ChIP) |
Developmental Studies Hybridoma Bank |
Cat#GFP-12E6 |
| GFP (for ChIP) |
Developmental Studies Hybridoma Bank |
Cat#GFP-8H11 |
| Chemicals, Peptides, and Recombinant Proteins |
| Auxin (3-Indole-acetic acid sodium salt) |
Abcam |
Cat#ab146403 |
| JQ1 |
Tocris |
Cat#4499 |
| dBET6 |
Selleckchem |
Cat#S876202 |
| NVP-2 |
MedChemExpress |
Cat#HY-12214A |
| THAL-SNS-032 (dCDK9) |
MedChemExpress |
Cat#HY-123937 |
| Doxycycline |
Stem Cell Technologies |
Cat#72742 |
| Benzonase |
Millipore Sigma |
Cat#E1014 |
| Dynabeads Protein G |
Invitrogen |
Cat#10004D |
| ChromoTek GFP-Trap Magnetic Agarose |
Proteintech |
Cat#gtma |
| Deposited Data |
| Genomics data |
This study |
GSE232869 |
| Experimental Models: Cell Lines |
| DLD-1 |
ATCC |
Cat#CCL-221 |
| NCI-H2009 |
ATCC |
Cat#CRL-5911 |
| Mouse embryonic fibroblasts |
STEMCELL Technologies |
Cat#00325 |
| BRD4-IAA7 DLD-1 #G3/D4 |
This study |
NA |
| Recombinant DNA |
| Cas9 plasmid for BRD4-IAA7 (sgRNA: CACCGAATCTTTTCTGAGCGCACCT) |
Zheng et al., 2021
|
NA |
| Cas9 plasmid for BRD4-IAA7 (sgRNA: CACCGATCAAAGTCAGAAGCCACCT) |
Zheng et al., 2021
|
NA |
| BRD4-IAA7-Neo donor plasmid |
This study |
pBZ14 |
| BRD4-IAA7-Hygro donor plasmid |
This study |
pBZ15 |
| Lenti-TetON-Sox2 |
Addgene |
Cat#110280 |
| p6344 pcDNA4-TO-HA-Brd4FL |
Addgene |
Cat#31351 |
| pFlag-CMV2-Brd4 del ET |
Addgene |
Cat#21938 |
| pTet-On-sNLS-3xFlag-EGFP-UBC-rtTeR-IRES-BSD |
This study |
pBZ113 |
| pTet-On-sNLS-3xFlag-BRD4-FL-UBC-rtTeR-IRES-BSD |
This study |
pBZ111 |
| pTet-On-sNLS-3xFlag-BRD4-ΔBD-UBC-rtTeR-IRES-BSD |
This study |
pBZ160 |
| pTet-On-sNLS-3xFlag-BRD4-ΔET-UBC-rtTeR-IRES-BSD |
This study |
pBZ136 |
| pTet-On-sNLS-3xFlag-BRD4-ΔCTM-UBC-rtTeR-IRES-BSD |
This study |
pBZ158 |
| pTet-On-sNLS-3xFlag-BRD4S-UBC-rtTeR-IRES-BSD |
This study |
pBZ137 |
| pTet-On-sNLS-3xFlag-BRD4-ΔN-UBC-rtTeR-IRES-BSD |
This study |
pBZ176 |
| pTet-On-sNLS-3xFlag-BRD4-Cm-UBC-rtTeR-IRES-BSD |
This study |
C1 |
| pTet-On-sNLS-3xFlag-BRD4-C-UBC-rtTeR-IRES-BSD |
This study |
C2 |
| pTet-On-sNLS-3xFlag-BRD4-Cs-UBC-rtTeR-IRES-BSD |
This study |
C3 |
| pTet-On-sNLS-GFP-UBC-rtTeR-IRES-BSD (Vector) |
This study |
MS1 |
| pTet-On-sNLS-GFP-BRD4-FL-UBC-rtTeR-IRES-BSD |
This study |
MS0 |
| pTet-On-sNLS-GFP-BRD4-CTM-UBC-rtTeR-IRES-BSD |
This study |
MS2 |
| pTet-On-sNLS-GFP-BRD4-CsΔCTM-UBC-rtTeR-IRES-BSD |
This study |
MS3 |
| pTet-On-sNLS-GFP-BRD4-Cs-UBC-rtTeR-IRES-BSD |
This study |
MS4 |
| pTet-On-sNLS-GFP-BRD4-C-UBC-rtTeR-IRES-BSD |
This study |
MS6 |
| pTet-On-sNLS-GFP-BRD4-FL-ΔBD-UBC-rtTeR-IRES-BSD |
This study |
MS7 |
| pTet-On-sNLS-GFP-BRD4-FL-ΔCd-UBC-rtTeR-IRES-BSD |
This study |
pBZ196 |
| pTet-On-sNLS-GFP-BRD4-FL-ΔCp-UBC-rtTeR-IRES-BSD |
This study |
pBZ197 |
| Software and Algorithms |
| bowtie 2.2.6.0 |
Langmead et al., 2009
|
NA |
| TopHat 2.1.0 |
Trapnell et al., 2009
|
NA |
| DESeq2 |
Love et al., 2014
|
NA |
| deepTools 3.1.1 |
Ramírez et al., 2016
|
NA |
| featureCounts 2.0.1 |
Liao et al., 2014
|
NA |
| AlphaFold integrated in ChimeraX |
Pettersen et al., 2021
|
NA |
