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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Adv Drug Deliv Rev. 2023 Jun 28;199:114978. doi: 10.1016/j.addr.2023.114978

Figure 8.

Figure 8.

TIRF and PALM/STORM imaging of HeLa exosomes. (A) (a) Schematic illustration of indirect IF labeling of CD63 and exosome membrane stained with CM-Dil. (b) TIRF image of HeLa-derived exosomes stained with CM-Dil. (c) IF labeling of CD63 with Alexa Fluor 647, conventional diffractionlimited image (upper right) and PALM/STORM image (low left). (d) Immunofluorescence control sample without primary antibody for specificity detection, CM-Dil channel. (e) Immunofluorescence control sample without primary antibody for specificity detection, Alexa Fluor 647 channel. (B) Colocalization of SKBR3 exosomes and MRC-5 lysosomes. (a) TIRF image of internalized SKBR3 exosomes (red) and MRC-5 membrane (blue). (b) PALM/STORM image of the same region as shown in (a). (c) TIRF image of MRC-5 lysosomes (green) and MRC-5 membrane (blue). (d) PALM/STORM image of the same region as shown in (c). (e) Colocalization of MRC-5 lysosomes (green) and internalized SKBR3 exosomes (red). Fig. 8 reproduced from Refs [130].