Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Gαi3 (C-10) | Santa Cruz Biotechnology | #sc-262 |
| FLAG M2 | Millipore Sigma | F1804 |
| Glu-Glu-epitope Tag | Millipore Sigma | AB3788 |
| Gαo (A2) | Santa Cruz Biotechnology | #sc-13532 |
| Gαs/olf (C-18) | Santa Cruz Biotechnology | #sc-383 |
| HA Tag | Roche | #11583816001 |
| Myc-Tag (9B11) Antibody | Cell Signaling Technology | #2276 |
| polyHistidine | Millipore Sigma | H1029 |
| α-Tubulin (DM1A) | Millipore Sigma | CP06 |
| β-Actin | LI-COR | #926-42212 |
| GAIP | 77 | N/A |
| Gβ (H-1) | Santa Cruz Biotechnology | #sc-166123 |
| GINIP (goat) | Santa Cruz Biotechnology | #sc-247284 |
| Gαq (E-17) | Santa Cruz Biotechnology | #sc-393 |
| β3 Tubulin (Tuj1) (2G10) | Santa Cruz Biotechnology | #sc-80005 |
| GFAP clone GA5 | Millipore Sigma | MAB360 |
| GINIP (rabbit) | Aviva | ARP70657_P050 |
| SYP/Synaptophysin (H-8) | Santa Cruz Biotechnology | #sc-55507 |
| vGlut1 (A-8) | Santa Cruz Biotechnology | #sc-377425 |
| GAD65 | Developmental Studies Hybridoma Bank | AB_528264 |
| PSD95 (7E3-1B8) | Abcam | #ab13552 |
| Gephyrin | Synaptic Systems | #147011 |
| Goat anti-rabbit Alexa Fluor 680 | Invitrogen | #A21077 |
| Goat anti-mouse Alexa Fluor 680 | Invitrogen | #A21058 |
| Goat anti-mouse IRDye 800 | LI-COR | #926-32210 |
| Goat anti-rabbit DyLight 800 | Thermo | #35571 |
| Donkey anti-goat IRDye 680RD | LI-COR | #926-68074 |
| Goat anti-mouse Alexa Fluor 488 | Invitrogen | #A11017 |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | #A11070 |
| Goat anti-mouse Alexa Fluor 594 | Invitrogen | #A11020 |
| Goat anti-rabbit Alexa Fluor 594 | Invitrogen | #A11072 |
| Bacterial and Virus Strains | ||
| NEB 5-alpha competent E. coli | NEB | Cat# C2987I |
| BL21(DE3) Chemically Competent E. coli | Invitrogen | Cat# C600003 |
| AAV-hSyn-Cre | Addgene | Addgene Cat# 105553-AAV1 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Forskolin | Tocris | Cat# 1099 |
| GABA | Tocris | Cat# 0344 |
| Brimonidine | Ark Pharm | Cat# AK-35795 |
| Dopamine HCl | Alfa Aesar | Cat# A11136 |
| CGP 54626 hydrochloride | Tocris | Cat# 1088 |
| Yohimbine | Alfa Aesar | Cat# J60185 |
| (+) - Bicuculine | TCI | Cat# B1890 |
| (R)-Baclofen | Tocris | Cat# 0796 |
| DL-isoproterenol hydrochloride | Alfa Aesar | Cat# J61788 |
| Tetrodotoxin citrate | Cayman | Cat# 18660-81-6 |
| CNQX disodium salt | Tocris | Cat# 1045 |
| DL-AP5 Na salt | Tocris | Cat# 3693 |
| Polyethylenimine (PEI) | Polysciences | Cat# 23966-1 |
| Q5 DNA polymerase | New England Biolabs | Cat# M0491L |
| SigmaFAST protease inhibitor cocktail | Sigma | Cat# S8830 |
| Critical Commercial Assays | ||
| NanoGlo Luciferase Assay System | Promega | Cat# N1120 |
| LANCE cAMP kit | PerkinElmer | Cat# AD0262 |
| RNAscope 2.0 Assay kit | Bio-techne | Cat# 320850 |
| Deposited Data | ||
| Original images from blotting and immunofluorescence staining | This paper | DOI: 10.17632/3ng65trfc7.1 |
| Experimental Models: Cell Lines | ||
| Human: 293T | ATCC | Cat# CRL-3216 |
| Human: Lenti-X 293T | Takara Bio | Cat# 632180 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | Charles River | Strain code: 027 |
| Mouse: C57BL/6N-Atm1Brd/a Phf24tm1a(EUCOMM)Hmgu/BcmMmucd | Mutant Mouse Resource & Research Centers (MMRRC) | #037754-UCD |
| Mouse: B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J | The Jackson Laboratory | #016226 |
| Mouse: B6.129S2-Emx1tm1(cre)Krj/J | The Jackson Laboratory | #005628 |
| Mouse: B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/MwarJ | The Jackson Laboratory | #028862 |
| Recombinant DNA | ||
| pLIC-His-GINIP | This paper | N/A |
| pLIC-His-RGS4 | This paper | N/A |
| pLIC-GST-GINIP | This paper | N/A |
| pET28b-Gαi3 (rat) | 78 | N/A |
| pGEX-4T-1-Gαi3 (rat) | 78 | N/A |
| pLIC-Gαi1(int.6xHis) | 35 | N/A |
| pET28b-Gαi2 | 79 | N/A |
| pET28b-Gαo | 79 | N/A |
| pET24a-Gαi3 (human) | 80 | N/A |
| pbb131-NMT | 81 | |
| pET28b-AC5 C1 | This paper | N/A |
| pET15b-hAC2 C2 | 82 | N/A |
| pGEX-GAIP | 83 | N/A |
| pGEX-KG-KB1753 | 83 | N/A |
| pHis6-Gαs | 84 | N/A |
| pGEX-4T-1-Gαi3/o chimera 1 (aka chimera 1 in this work) | 40 | N/A |
| pGEX-4T-1-Gai3/o chimera 3 (aka chimera 2 in this work) | 40 | N/A |
| pGEX-4T-1-Gai3/o chimera 4 (aka chimera 3 in this work) | 40 | N/A |
| pET28b-DAPLE CT (1650–2028) | 42 | N/A |
| p3xFLAG-CMV-14-GINIP | This paper | N/A |
| pcDNA3.1(+)-Gαz (EE-tagged) | cDNA Resource Center | GNA0Z0EI00 |
| pcDNA3-Gαq-HA | 85 | N/A |
| pcDNA3.1-Gα12-MYC | 86 | N/A |
| pcDNA3-YFP-hAC5 | 29 | N/A |
| pcDNA3.1(+)-FLAG-D2DR | 87 | N/A |
| pcDNA3-α2A-AR | 88 | N/A |
| pcDNA3.1(+)-GABABR1a | 89 | N/A |
| pcDNA3.1(+)-GABABR2 | 89 | N/A |
| pcDNA3.1-hRGS7 | 90 | N/A |
| pcDNA3.1-Gβ5 | 91 | N/A |
| pcDNA3.1-R7BP | 91 | N/A |
| pcDNA3.1-masGRK3ct-Nluc | 56 | N/A |
| pcDNA3.1-Nluc-EPAC-VV | 56 | N/A |
| pcDNA3.1-Venus(1–155)-Gγ2 (VN-Gγ2) | 55 | N/A |
| pcDNA3.1-Venus(155–239)-Gβ1 (VC-Gβ1) | 55 | N/A |
| pcDNA3.1(−)-3xHA-RGS8 | cDNA Resource Center | RGS080TN00 |
| pcDNA3-GAIP | 92 | N/A |
| psPAX2 | Addgene | Addgene cat# 12260 |
| pMD2.G | Addgene | Addgene cat# 12259 |
| pLenti-hSyn-Gβγ-BERKY1 | 63 | Addgene cat# 158429 |
| pLenti-hSynapsin-Nluc-EPAC-VV | This paper | N/A |
| P3VEA3–1-venus-arrestin-3 | 93 | N/A |
| α2A-AR-Rluc8 | 94 | N/A |
| D2R-Rluc8 | 94 | N/A |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Adobe Photoshop | Adobe | https://www.adobe.com/ |
| Adobe Illustrator | Adobe | https://www.adobe.com/ |
| pClamp 11 | Molecular Devices | https://www.moleculardevices.com/ |