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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Virology. 2023 Jun 13;585:109–116. doi: 10.1016/j.virol.2023.05.013

Figure 4. Comparing Fitness of Early Pandemic SARS-CoV-2 Mutants Using a Novel Infectious Clone.

Figure 4.

A) To assess the fitness of Spike D614G, wild-type (WT) and D614G were mixed at a 1:1 PFU ratio. After confirming the mix composition by Sanger sequencing and plaque assay, the mix was used to infect VeroE6 hACE2-TMPRSS2 cells. RNA was extracted from the viral supernatant at 1 dpi and used to synthesize cDNA downstream. B) Relative Fitness of SARS-CoV-2 USA-WA1/2020 wild-type infectious clone and a mutant bearing Spike D614G was calculated in VeroE6 hACE2-TMPRSS2 cells. Data represent two biological replicates, each consisting of three technical replicates, and the error bars represent the standard deviation from the mean. Statistical analysis was performed using a one-sample t-test with a null value of 1 (*** P = .0005).