Figure 1. In vivo electrophysiological recordings in the Pf during a reward tracking task.

(A) Left: Chronically implanted 16-channel electrode arrays were inserted into the right Pf of wild type mice. Right: Coronal sections through the thalamus and placement of electrode array into the Pf. Black arrows denote electrode tips. B) Schematic of reward tracking experimental design. Reflective markers were placed on the head (c and i) of water-deprived freely behaving mice and the reward spout (s) to track movements in relation to a 10% sucrose reward during wireless recordings of Pf neural activity. The right panel illustrates simultaneous neural recordings during acquisition of movement kinematic information from reflective markers in Cartesian space while mice tracked a reward lever. C) As mice followed the reward spout (target) left and right, they received rewards only when their head was close s to the target. Mice spent an average of 75% of their time following the target. D) The target moved along the horizontal axis with a full cycle taking approximately 5 seconds (top). See also Video S1.