| Antibodies |
|
|
|
| Anti-NeuroD1 |
Cell Signaling |
Cat# 4373S (D35G2); RRID: AB_1054907 |
| Anti-DNMTI |
Cell Signaling |
Cat# 5032S (D63A6); RRID: AB_1054819 |
| Anti-ASCLI |
Cell Signaling |
Cat# 43666S (E7N9C) |
| anti-p27 |
Cell Signaling |
Cat# 3686S (D69C12); RRID: AB_2077850 |
| anti-c-Myc |
Cell Signaling |
Cat# 5605S (D84C12); RRID: AB_1903938 |
| anti-CAD |
Cell Signaling |
Cat# 11933S; RRID: AB_2797772 |
| Anti-L-Myc |
Cell Signaling |
Cat# 76266S (E3M5P) |
| HRP-tagged anti-Rabbit secondary |
Cell Signaling |
Cat# 7074P2; RRID: AB_2099233 |
| HRP-tagged anti-Mouse secondary |
Cell signaling |
Cat# 7076P2; RRID: AB_330924 |
| Anti-DCK |
Santa Cruz Biotechnology |
Cat# sc-393098, Clone: H5 |
| anti-ASCLI |
Santa Cruz Biotechnology |
Cat# sc-374104; RRID: AB_1091856 |
| anti-NeuroD1 |
Abcam |
Cat# ab213725; RRID: AB_2801303 |
| anti-UCK2 |
Abcam |
Cat# ab104731; RRID: AB_1071246 |
| anti-CDA |
Abcam |
Cat# ab82347; RRID: AB_1658630 |
| FITC-anti-mouse CD4 antibody |
BD Biosciences |
Cat# 553046, Clone: RM4-5; RRID: AB_394582 |
| PerCP anti-mouse CD8 antibody |
BD Biosciences |
Cat# 561092, Clone 53–6.7; RRID: AB_1056168 |
| PE anti-mouse FoxP3 antibody |
BD Biosciences |
Cat# 560408, Clone: MF-23; RRID: AB_1645251 |
| PE-Cy7 anti-mouse CD11b antibody |
BD Biosciences |
Cat# 561098, Clone: M1/70; RRID: AB_2033994 |
| APC anti-mouse Ly6C antibody |
BD Biosciences |
Cat# 560595, Clone: AL-21; RRID: AB_1727554 |
| PE-CF594 anti-mouse Ly6G antibody |
BD Biosciences |
Cat# 562700, Clone: 1A8; RRID: AB_2737730 |
| anti-β-Actin antibody |
Sigma-Aldrich |
Cat# A5441; RRID: AB_476744 |
| Mouse IgG1 isotype control |
Abcam |
Cat# ab18443; RRID: AB_2736846 |
| Rabbit IgG isotype control |
Abcam |
Cat# ab172730; RRID: AB_2687931 |
| Anti-DNMT1 |
Active Motif |
Cat# 39204; RRID: AB_2614950 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Decitabine |
BOC Sciences |
Cat# 2353-33-5 |
| 5-Azacytidine |
BOC Sciences |
Cat# 320-67-2 |
| Teriflunomide |
MedChem Express |
Cat# HY-15405 |
| Fluorizoline |
Sigma-Aldrich |
Cat# SML2379 |
| Lipofectamine RNAiMax |
Invitrogen |
Cat# 1000144720 |
| Fast SYBR Green |
Applied Biosciences |
Cat# 4385612 |
| Pierce Rapid Gold BCA Protein Assay Kit |
Thermo-Scientific |
Cat# A53226
|
| Giemsa stain |
Sigma-Aldrich |
Cat # 48900 |
| 4–12% precast Tris-bis gradient gels |
Invitrogen |
Cat# NP0335 |
| Protein A/G plus agarose beads |
Santa Cruz Biotechnology |
Cat# sc-2003 |
| gentleMACS dissociator |
Miltenyi Biotec |
Cat# 130-093-235 |
| gentleMACS tubes |
Miltenyi Biotec |
Cat# 130-093-237 |
| 70-μm cell strainer |
Corning |
Cat# 352350 |
| eBioscience™ IC Fixation Buffer |
ThermoFisher Scientific |
Cat# 00-8222-49 |
| Monensin |
ThermoFisher Scientific |
Cat# 00-4505-51 |
| NuPAGE LDS Sample Buffer (4X) |
Invitrogen |
Cat# NP0007 |
| NuPAGE MOPS SDS Running Buffer |
Invitrogen |
Cat# NP0001 |
| NuPAGE Transfer Buffer |
Invitrogen |
Cat# NP0006 |
| Precision Plus Protein Standards |
Bio Rad |
Cat# 161-0394 |
|
| Critical commercial assays |
|
|
|
| RNeasy Mini Kit |
Qiagen |
Cat# 74104 |
| Cut & Tag Assay kit |
Active Motif |
Cat# 53165 |
| iScript cDNA Synthesis kit |
Bio-Rad |
Cat# 1708891 |
| FITC Annexin V Detection Kit Annexin |
BD Biosciences |
Cat# 556547 |
| MycoAlert™ Mycoplasma Detection Kit |
Lonza |
Cat# LT07-118 |
|
| Deposited data |
|
| RNA-Seq |
This paper |
GEO Accession ID: GSE238007
|
| Cut &Tag |
This paper |
GEO Accession ID: GSE238007
|
|
| Experimental models: Cell lines |
|
| F1339 |
Dr David MacPherson |
N/A |
| NCI-H82 |
ATCC |
HTB-175™
|
| NCI-H146 |
ATCC |
HTB-173 |
|
| Experimental models: Organisms/strains |
|
| Mouse B6/129SF1/J |
Jackson Laboratory |
RRID:IMSR_JAX:101043 |
|
| Oligonucleotides |
|
| DNMT1 siRNA |
Invitrogen |
Cat# 4390825 |
| Control siRNA |
Invitrogen |
Cat# 4390844 |
| Primers, see Table S6
|
Table S6
|
N/A |
|
| Software and algorithms |
|
| ImageJ |
NIH |
https://ImageJ.nih.gov/ij/
|
| FlowJo V10. |
BD Biosciences |
https://www.flowjo.com
|
| GraphPad Prism 7.0 |
GraphPad Software, Inc |
https://www.graphpad.com
|
| EASEQ |
Website |
https://easeq.net
|
| UseGalaxy |
Website |
https://usegalaxy.org
|
| Adobe Photoshop 2023 |
Adobe creative cloud |
https://www.adobe.com/creativecloud.html
|
