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. 2023 Sep 21;12(18):2328. doi: 10.3390/cells12182328

Figure 2.

Figure 2

Expression of p.Gln1171Ter C-Nap1 in NIH3T3 cell line. (A,B) Immunoblotting analysis using Myc (A) and CEP250 (B) antibodies. CEP250 WT was expressed at 255 kDa (full length) and ~160 kDa (short form). CEP250 p.Gln1171Ter was detected at ~150 kDa, corresponding to the early truncated protein. (C) Immunocytochemistry was performed in NIH3T3 cells transfected with wild-type CEP250 and p.Gln1171Ter. It was stained using γ-tubulin (red), Dapi (blue), and C-Myc (green) antibodies in NIH3T3 cells. White arrows point to centrosome.