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. 2023 Aug 24;45(9):7058–7074. doi: 10.3390/cimb45090446

Figure 4.

Figure 4

Inhibition of cell migration and 2D invasion in MDA-MB-231 by SJP1602. (A) Cell migration capacity was analyzed using the transwell migration assay. MDA-MB-231 cells were seeded in the 24-well chamber and treated with vehicle or FAK inhibitors (SJP1602, VS-6063, GSK2256098) at a concentration of 5 μM. After 16 h, migrated cells were analyzed using a microscope. (B) Bar diagram illustrates the relative cell migration of MDA-MB-231 cells treated with FAK inhibitors, including SJP1602, VS-6063, and GSK2256098. Statistical analysis was performed using an unpaired, two-tailed Student’s t-test, and * p < 0.05, *** p < 0.001 denote significant differences in cell migration compared to the control group. (C) Cell invasion capacity was analyzed using the Boyden chamber assay. MDA-MB-231 cells were seeded in the 24-well Boyden chambers with Matrigel-coated filters and treated with vehicle or FAK inhibitors (SJP1602, VS-6063, GSK2256098) at a concentration of 5 μM. After 48 h, cells that invaded the Matrigel were stained using crystal violet and analyzed using a microscope. (D) Bar diagram representing the number of invaded cells into the Matrigel, stained with crystal violet. Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test. ** p < 0.01 and *** p < 0.001 indicate significant differences in 2D invasion compared to the control group.