Figure 5.

Inhibition of 3D invasion of TNBC cells by SJP1602. (A) The figure illustrates the impact of SJP1602 on the invasion of 3D single spheroids formed by MDA-MB-231. Cells were added to an ultra-low 96-well round-bottom plate and cultured for 3 days to form spheroids. The medium was removed, and a mixture of neutralized Type I Collagen and Matrigel was added to the wells. After 1 h of incubation, cells were treated with vehicle or FAK inhibitors (SJP1602, VS-6063, GSK2256098) at concentrations of 1 μM or 5 μM. The plate was then incubated for 72 h. Relative invasion was quantified for MDA-MB-231 (B), and other TNBC cell lines, including 4T1 (C) and Hs578T (D). Statistical analysis was performed using an unpaired, two-tailed Student’s t-test, and * p < 0.05, ** p < 0.005 denote significant differences in cell invasion compared to the control group.