Antibodies |
Mouse monoclonal Anti-cyclobutane pyrimidine dimers |
Cosmo Bio |
Cat# NM-DND-001 |
Rabbit anti-active Caspase-3 |
BD Biosciences |
Cat# 559565 |
Alexa Fluor 546 goat anti-mouse IgG |
ThermoFisher |
Cat# A-11030 |
Alexa Fluor 594 goat anti-rabbit IgG |
ThermoFisher |
Cat# A-11012 |
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Biological samples |
Danio rerio embryos |
This paper |
N/A |
Danio rerio larvae |
This paper |
N/A |
Danio rerio ovaries |
This Paper |
N/A |
O. latipes embryos |
This Paper |
N/A |
I. punctatus ovaries |
This Paper |
N/A |
Chemicals, peptides, and recombinant proteins |
DAPI |
Sigma-Aldrich |
Cat# D9542 |
Methanol |
Sigma-Aldrich |
Cat# 34860 |
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Critical Commercial Assays |
QuanTiTect Reverse Transcription Kit |
Qiagen |
Cat# 205311 |
PowerUp SYBR Green Master Mix |
ThermoFisher |
Cat# A25742 |
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Deposited data |
Code for bioinformatic exploration of loss of gadusol |
This paper |
https://github.com/nclark-lab/gadusol
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RNAseq data (raw and analyzed) |
GEO |
GSE229587 |
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Experimental models: Organisms/strains |
Zebrafish D. rerio (Tübingen strain) |
ZIRC |
ZL57 |
Zebrafish D. rerio (AB strain) |
ZIRC |
ZL1 |
Zebrafish D. rerio (mitfaw2/w2) |
ZIRC |
ZL2104 |
Zebrafish D. rerio (eevs zj2/zj2) |
This paper |
N/A |
Zebrafish D. rerio (eevs zj5/zj5) |
This paper |
N/A |
Oligonucleotides |
Primers for sequences cloning, see Table S1
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This paper |
N/A |
Primers for qRT-PCR, see Table S1
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This paper |
N/A |
Software and algorithms |
GraphPad |
GraphPad Software |
https://graphpad.com
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ImageJ |
NIH |
https://imagej.nih.gov/ij/
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Microsoft Excel Data Analysis Student t test |
Microsoft |
N/A |
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Other |
Benchtop UV Transilluminator |
UVP |
M-15V P/N 95–0456-01 |
UVB broad band bulb (306nm) |
Ushio |
G8T5E |
Digital UV Radiometer UVB |
Solarmeter |
6.0 UVB |
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