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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: Cell Rep. 2023 Jul 11;42(7):112711. doi: 10.1016/j.celrep.2023.112711

Figure 1. Large-panel neutralization breadth and potency for PGT145 and PG9RSH variants.

Figure 1.

Neutralization breadth and potency of PGT145 and PG9RSH variants assayed on a panel of 208 pseudoviruses.

(A and B) Breadth/potency curves for PGT145 and PG9RSH variants and controls, respectively. Curves represent the fraction of pseudoviruses that were neutralized with IC50 smaller than the given cutoff. An increase in breadth and potency is indicated by a shift upward and left. PGT145 variant DU303 and PG9RSH variants N(100f)Y and DU025 improve breadth and potency relative to wild type. For IC80 curves, see Figure S3.

(C) Breadth/potency curves for PG9RSH DU025 and PGDM1400. Despite slightly weaker median and mean neutralization potency, the overall breadth and potency of DU025 rival that of the best-in-class PGDM1400 antibody.

(D–F) Neutralization dendrograms for variants DU303, PG9RSH N(100f)Y, and DU025, and respectively. Pseudoviruses are grouped into clades by sequence similarity, forming a tree graph. Internal branches in the tree, which denote groups of viruses, are colored gray. Terminal branches, corresponding to a single pseudovirus, are colored by IC50, where high neutralization potency is indicated by warm colors, low potency is indicated by cool colors, and lack of neutralization is indicated by gray.

(G) Summary of large-panel neutralization breadth and potency for variants and controls, measured by IC80.