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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: Cell Rep. 2023 Jun 20;42(7):112676. doi: 10.1016/j.celrep.2023.112676

Figure 1. Single-cell RNA-seq profiling of Arabidopsis infected with Pseudomonas syringae.

Figure 1.

(A) Confocal micrograph of a representative image of an Arabidopsis leaf 24 h post-infiltration (hpi) with mCherry-tagged Pst DC3000 ΔhopQ1 (Pst DC3000). The image is a maximum projection from 21 confocal z stacks. Chlorophyll autofluorescence is shown in gray. Scale bar: 100 μm.

(B and C) Pst DC3000 colony number and area in infiltrated leaf tissue shown in (A). Boxplot shows median with minimum and maximum values indicated (n = 10 images from 4 plants).

(D) Overview of the scRNA-seq experiment. Four-week-old Arabidopsis Col-0 was infiltrated with Pst DC3000 or 10 mM MgCl2. Twenty-four hours post-infiltration, protoplasts were prepared using the Tape-Arabidopsis Sandwich method. Cells were isolated on the 10X Genomics Chromium chip and sequenced using the Illumina NovaSeq6000 platform.

(E and F) Single-cell uniform manifold approximation and projection (UMAP) plots from both Pst DC3000 and mock-treated samples, colored according to cluster identities (E) and cell types (F). M, mesophyll; B, bundle sheath; P, parenchyma and procambium; G, guard cells; C, companion cells.

See also Figures S1 and S2 and Tables S1A and S2.