KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| Pseudomonas syringae pv. tomato DC3000 | Cuppels76 | N/A |
| Pseudomonas syringae pv. tomato DC3000 ΔhopQ1 | Wei et al.28 | N/A |
| Pseudomonas syringae pv. tomato DC3000 ΔhopQ1 - 3xmCherry | This study | N/A |
| Pseudomonas syringae pv. tomato DC3000 - 3xmCherry | This study | N/A |
| Escherichia coli strain DH5a | Fisher | Cat# 11319019 |
| Agrobacterium tumefaciens strain GV3101 | Coppinger et al.84 | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Cellulase Onuzuka R10 | Yakult | Cat# L0012 |
| Macerozyme R10 | Yakult | Cat# L0021 |
| Mannitol | Sigma | Cat# M4125-5KG |
| MES (2-(N-Morpholino)ethanesulfonic acid hydrate) | Fisher | Cat# BP300-100 |
| Bovine Serum Albumin (BSA) | Fisher | Cat# BP1600-100 |
| Sucrose | Fisher | Cat# BP220-212 |
| Tween-20 | Fisher | Cat# BP337-500 |
| Silwet-77 | Bioworld (Fisher) | Cat# NC0138454 |
| β-mercaptoethanol | Amersco (VWR) | Cat# M131-100ML |
| KCl | Mallinckrodt | Cat# 6858 |
| MS medium | RPI (Fisher) | Cat# 50-213-423 |
| Trizol | Invitrogen (Fisher) | Cat# 15596018 |
| Dulbecco’s Phosphate-buffered saline (DPBS, 1X) | Cellgro | Cat# 21-031-CV |
| Xylitol | Fisher | Cat# AAA1694422 |
| Sodium deoxycholate | Sigma | Cat# D6750-500G |
| Urea | Fisher | Cat# U15-3 |
| Paraformaldehyde | Sigma | Cat# P6148 |
| Rifampicin | Fisher | Cat# BP2679-5 |
| Spectinomycin | Sigma | Cat# 56757010GM |
| Hygromycin | Gibco (Fisher) | Cat# 10687010 |
| Carbenicillin | Fisher | Cat# BP2648-5 |
| MgCl2 | Fisher | Cat# M35-212 |
| Sodium Hydroxide | Fisher | Cat# S230-500 |
| Critical commercial assays | ||
| In-Fusion HD Cloning Plus | Clontech/Takara | Cat# 638910 |
| pENTR Directional TOPO Cloning kit | Invitrogen | Cat# K2400-20 |
| Gateway Cloning Technology LR | Invitrogen | Cat# 11791-020 |
| RQ1 RNase-Free DNase | Promega | Cat# M6101 |
| Chromium Single Cell 3’ reagent kit V3 | 10X Genomics | Cat# PN-1000268 |
| QuantSeq FWD kit | Lexogen | Cat# 015 |
| Kapa Library Quantification kit | Kapa Biosystems/Roche | Cat# 07960484001 |
| Deposited data | ||
| scRNA-seq and bulk RNA-seq datasets | This study | GSE213625 |
| Original microscopy images | This study | https://doi.org/10.5281/zenodo.7686553 |
| Original code | This study | https://doi.org/10.5281/zenodo.7888124 |
| Experimental models: Organisms/strains | ||
| Arabidopsis thaliana: Col-0 wild-type | ABRC (Arabidopsis Biological Resource Center) | CS70000 |
| Arabidopsis: pFRK1::NLS-3xmVENUS | Zhou et al.46 | N/A |
| Arabidopsis: pLipop1::NLS-3xmCitrine (Line 1, 19-10-5) | This study | ABRC: CS73265 |
| Arabidopsis: pLipop1::NLS-3xmCitrine (Line 2, 19-15-1) | This study | ABRC: CS73266 |
| Arabidopsis: pCBP60g::NLS-3xmCitrine (Line 1, 22-4) | This study | ABRC: CS73260 |
| Arabidopsis: pCBP60g::NLS-3xmCitrine (Line 2, 22-1) | This study | ABRC: CS73408 |
| Arabidopsis: pEXPA10::NLS-3xmCitrine (Line 1, 16-7-3) | This study | ABRC: CS73261 |
| Arabidopsis: pEXPA10::NLS-3xmCitrine (Line 2, 16-8) | This study | ABRC: CS73262 |
| Arabidopsis: pPIP1;4::NLS-3xmCitrine (Line 1, 23-4) | This study | ABRC: CS73267 |
| Arabidopsis: pPIP1;4::NLS-3xmCitrine (Line 2, 23-5) | This study | ABRC: CS73268 |
| Arabidopsis: pILL5::NLS-3xmCitrine (Line 1, 4-5-1) | This study | ABRC: CS73263 |
| Arabidopsis: pILL5::NLS-3xmCitrine (Line 2, 4-1-3) | This study | ABRC: CS73264 |
| Oligonucleotides | ||
| All primers are listed in Table S3 | This study | N/A |
| Recombinant DNA | ||
| pLipop1::NLS-3xmCitrine | This study | Addgene: 192522 |
| pCBP60g::NLS-3xmCitrine | This study | Addgene: 192521 |
| pPIP1;4::NLS-3xmCitrine | This study | Addgene: 192524 |
| pILL5::NLS-3xmCitrine | This study | Addgene: 192525 |
| pEXPA10::NLS-3xmCitrine | This study | Addgene: 192523 |
| Software and algorithms | ||
| Leica Application Suite X (v3.4.2.18368) | Leica Microsystems | https://www.leica-microsystems.com/ |
| Zeiss Zen 2.3 SP1 FP1 (v14.0.12.201) | Zeiss | https://www.zeiss.com/corporate/int/home.html |
| GraphPad Prism (v9.2.0) | GraphPad | https://www.graphpad.com |
| Imaris (v8.0) | Oxford Instruments Imaris | https://imaris.oxinst.com/ |
| Geneious Prime (v2021.1.1) | Geneious | www.geneious.com |
| R/RStudio (v1.4.1103) | R CoreTeam77 RStudio Team78 | https://www.r-project.org/ |
| Seurat (v3.9.9005) | Hao et al.79 | https://github.com/satijalab/seurat |
| TrimGalore | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ | https://github.com/FelixKrueger/TrimGalore |
| EdgeR (v3.12) | McCarthy et al.80 Robinson et al.81 | https://bioconductor.org/packages/release/bioc/html/edgeR.html |
| Cellranger (v6.0.1) | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation |
| Velocyto (v0.17.15) | La Manno et al.72 | http://velocyto.org/ |
| Monocle 3 (v1.0.0) | Trapnell et al.37 Qiu et al.40 | https://github.com/cole-trapnell-lab/monocle3 |
| DESeq2 (v1.30.1) | Love et al.82 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| topGO (v3.12) | Alexa and Rahnenfuhrer83 | https://bioconductor.org/packages/release/bioc/html/topGO.html |