Fig. 1. OBOX expression in mouse oocytes and preimplantation embryos.

a, Top 250 TFs based on the translation levels (RPF, ribosome protected fragments)²⁵ or motif enrichment in all distal accessible regions or those near ZGA genes based on ATAC-seq²⁶ (left) in embryos. PRD-like homeobox family (red), nuclear receptor (NR) (blue), and Kruppel-like factors (KLF) (green) TFs and their ranks are indicated. OBOX, OTX2, GSC, CRX, and PITX1 binding motifs are shown (right). E2C, early 2-cell; L2C, late 2-cell. b, Line plots showing mRNA²⁸ and translation²⁵ levels of maternal, minor, and major ZGA Obox in oocytes and early embryos (2 biological replicates). c, Sequence alignment of OBOX proteins based on Clustal Omega.