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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Nature. 2023 Jul 17;620(7976):1047–1053. doi: 10.1038/s41586-023-06428-3

Extended Data Fig. 5 |. Maternal and zygotic OBOX redundantly support early development.

Extended Data Fig. 5 |

a, Expression of stage-specific genes in WT, Obox mutant, and rescued embryos. BL*2C, Obox mzKO embryos arrested at 2C when WT developed to blastocyst. b, Schematic of OBOX3 rescue in Obox mzKO embryos with embryo morphology and developmental rates shown (3 biological replicates). Scale bar, 100 μm. c-d, OBOX4 expression (c), embryo morphology, and developmental rate (d) with or without Obox4 rescue (3 biological replicates). Scale bar, 75 μm. e, RNA-seq showing Obox levels in WT and maternal Obox knockout embryos. Check and cross, the presence or absence of Obox mRNAs. f, OBOX3 immunofluorescence in WT and Obox mKO embryos (3 biological replicates). Scale bar, 20 μm. g-h, Embryo morphology, developmental rate (g), and expression of stage-specific genes (h) for WT and Obox mKO embryos in vivo at the blastocyst stage (2 biological replicates). Scale bar, 100 μm. i, Obox expression levels in Obox mutant embryos.