Extended Data Fig. 2 |. OBOX protein levels in oocytes and early embryos.

a-b, Line plots showing Obox mRNA and translation levels during oocyte maturation (2 biological replicates) and early embryo development (2 biological replicates) based on datasets from the previous publications^30,31. NA, data not available. c, OBOX antibody epitope locations. d, Immunofluorescence showing OBOX signals detected by OBOX antibodies upon Flag-OBOX-GFP overexpression in mESCs (2 biological replicates). Scale bar, 10μm. e-h, OBOX immunofluorescence in mouse oocytes and embryos (3 biological replicates). BL, blastocyst. Scale bar, 20 μm.