Supplementary Table 1. Relative expression of clock genes, energy-sensing and metabolism-related genes in rat liver on restricted feeding schedules.
| Gene | Liver | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CG | DF | RF-n | RF-d | |||||||||
| ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | |
| Clock | 1.1 ± 0.1 | 0.5 ± 0.1 | 1.1 ± 0.1 | 0.5 ± 0.1 | 0.6 ± 0.1 | 1.8 ± 0.3 | 0.9 ± 0.1 | 0.4 ± 0.1 | 1.0 ± 0.1 | 0.7 ± 0.2 | 0.7 ± 0.1 | 1.0 ± 0.1 |
| 1.0 | 0.5 | 1.2 | 0.5 | 0.6 | 1.8 | 0.9 | 0.4 | 1.0 | 0.7 | 0.7 | 1.0 | |
| [1.0-1.2] | [0.4-0.6] | [1.0-1.3] | [0.4-0.6] | [0.5-0.8] | [1.6-2.1] | [0.8-1.0] | [0.3-0.5] | [0.9-1.0] | [0.6-0.9] | [0.6-0.8] | [0.9-1.0] | |
| Bmal1 | 1.0 ± 0.3 | 0.03 ± 0.01 | 0.4 ± 0.1 | 0.03 ± 0.01 | 0.5 ± 0.1 | 0.9 ± 0.3 | 0.8 ± 0.1 | 0.02 ± 0.01 | 0.6 ± 0.1 | 0.04 ± 0.02 | 0.8 ± 0.1 | 1.6 ± 0.2 |
| 1.0 | 0.03 | 0.4 | 0.03 | 0.4 | 0.9 | 0.8 | 0.01 | 0.6 | 0.04 | 0.8 | 1.6 | |
| [0.8-1.3] | [0.03-0.04] | [0.3-0.5] | [0.03-0.04] | [0.4-0.5] | [0.7-1.0] | [0.7-0.9] | [0.01-0.02] | [0.6-0.7] | [0.02-0.04] | [0.7-0.9] | [1.5-1.8] | |
| Silt1 | 1.1 ± 0.3 | 0.9 ± 0.3 | 0.6 ± 0.2 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.8 ± 0.3 | 0.9 ± 0.1 | 2.0 ± 0.9 | 0.4 ± 0.1 | 3.5 ± 1.9 | 0.6 ± 0.3 | 0.7 ± 0.2 |
| 1.0 | 0.8 | 0.6 | 0.5 | 0.4 | 0.8 | 1.0 | 1.8 | 0.3 | 3.0 | 0.6 | 0.7 | |
| [0.8-1.4] | [0.6-1.2] | [0.4-0.7] | [0.4-0.6] | [0.4-0.5] | [0.6-0.9] | [0.8-1.0] | [1.4-2.8] | [0.3-0.5] | [2.0-4.7] | [0.4-0.9] | [0.6-0.8] | |
| Ampk | 1.1 ± 0.3 | 1.4 ± 0.4 | 1.5 ± 0.3 | 3.0 ± 0.8 | 2.0 ± 0.6 | 1.4 ± 0.6 | 0.9 ± 0.2 | 2.2 ± 0.5 | 0.9 ± 0.2 | 2.9 ± 0.7 | 0.8 ± 0.1 | 1.3 ± 0.4 |
| 1.0 | 1.3 | 1.6 | 3.0 | 1.9 | 1.4 | 1.0 | 2.1 | 0.9 | 3.1 | 0.7 | 1.3 | |
| [0.9-1.4] | [1.1-1.6] | [1.2-1.8] | [2.3-3.5] | [1.6-2.6] | [0.8-1.9] | [0.8-1.1] | [1.7-2.6] | [0.8-1.0] | [2.2-3.4] | [0.6-0.9] | [1.0-1.6] | |
| Nampt | 1.0 ± 0.2 | 2.1 ± 0.7 | 5.4 ± 0.7 | 3.9 ± 1.2 | 2.7 ± 1.0 | 1.9 ± 0.8 | 0.9 ± 0.3 | 6.5 ± 1.5 | 3.9 ± 1.1 | 8.2 ± 2.3 | 1.5 ± 0.5 | 2.1 ± 1.4 |
| 1.0 | 2.1 | 5.6 | 3.8 | 2.4 | 1.6 | 0.8 | 7.0 | 3.7 | 8.3 | 1.4 | 1.7 | |
| [0.8-1.2] | [1.6-2.5] | [4.7-6.0] | [3.5-4.5] | [1.7-3.6] | [1.1-2.8] | [0.7-1.1] | [5.1-7.7] | [3.0-4.8] | [6.0-10.2] | [1.2-1.7] | [1.1-3.3] | |
| Pparg | 1.0 ± 0.3 | 1.6 ± 0.4 | 0.9 ± 0.4 | 1.0 ± 0.3 | 0.9 ± 0.3 | 1.7 ± 0.5 | 0.9 ± 0.3 | 1.5 ± 0.4 | 0.9 ± 0.5 | 2.7 ± 1.1 | 1.1 ± 0.4 | 1.5 ± 0.3 |
| 1.0 | 1.7 | 1.0 | 1.0 | 0.7 | 1.6 | 0.9 | 1.5 | 0.7 | 2.6 | 1.2 | 1.4 | |
| [0.7-1.3] | [1.4-1.8] | [0.6-1.2] | [0.8-1.4] | [0.7-1.0] | [1.2-2.2] | [0.7-1.1] | [1.2-1.8] | [0.6-1.3] | [2.0-3.3] | [0.6-1.2] | [1.2-1.8] | |
| Pgc1a | 1.1 ± 0.4 | 2.1 ± 1.1 | 2.2 ± 0.6 | 1.8 ± 0.5 | 1.9 ± 0.5 | 4.0 ± 1.8 | 3.9 ± 0.7 | 9.6 ± 3.4 | 3.0 ± 1.2 | 16.4 ± 9.2 | 2.7 ± 0.9 | 7.0 ± 3.1 |
| 1.0 | 1.6 | 2.0 | 1.7 | 1.7 | 4.3 | 4.1 | 10.1 | 2.7 | 15.5 | 2.5 | 7.3 | |
| [0.7-1.5] | [1.4-3.0] | [1.8-2.7] | [1.4-2.4] | [1.6-2.5] | [2.1-5.6] | [3.1-4.5] | [6.6-12.2] | [2.0-4.1] | [7.5-24.1] | [2.2-2.9] | [5.1-8.0] | |
| Ucp2 | 0.9 ± 0.3 | 0.9 ± 0.2 | 0.5 ± 0.06 | 0.5 ± 0.2 | 0.6 ± 0.1 | 0.6 ± 0.2 | 0.6 ± 0.2 | 0.9 ± 0.2 | 0.3 ± 0.1 | 1.2 ± 0.3 | 0.4 ± 0.1 | 0.4 ± 0.1 |
| 1.0 | 0.9 | 0.5 | 0.5 | 0.6 | 0.5 | 0.6 | 1.0 | 0.3 | 1.1 | 0.4 | 0.4 | |
| [0.6-1.1] | [0.7-1.1] | [0.5-0.6] | [0.4-0.6] | [0.5-0.7] | [0.4-0.7] | [0.4-0.7] | [0.8-1.1] | [0.2-0.4] | [0.9-1.6] | [0.4-0.5] | [0.3-0.6] | |
Data (2-ΔΔCt) are shown as mean ±; standard deviation, median and [interquartile interval]. CG: control group, DF: day-fed, RF-n: restricted night-fed, and RF-d: restricted day-fed. ZT3, ZT11 and ZT17: Zeitgeber times 3, 11 and 17, corresponding to 0900, 1700, and 2300h, respectively.