Supplementary Table 2. Relative expression of clock genes, energy-sensing and metabolism-related genes in rat brown adipose tissue on restricted feeding schedules.
| Gene | Brown adipose tissue | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CG | DF | RF-n | RF-d | |||||||||
| ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | |
| Clock | 1.0 ± 0.2 | 1.0 ± 0.3 | 1.0 ± 0.1 | 1.0 ± 0.2 | 1.0 ± 0.7 | 1.2 ± 0.2 | 1.0 ± 0.7 | 0.6 ± 0.2 | 0.8 ± 0.1 | 1.2 ± 0.6 | 1.0 ± 0.5 | 0.8 ± 0.2 |
| 1.0 | 0.9 | 1.0 | 1.0 | 0.9 | 1.3 | 0.6 | 0.5 | 0.8 | 0.9 | 0.8 | 0.7 | |
| [0.8-1.2] | [0.8-1.2] | [0.9-1.1] | [0.7-1.1] | [0.7-1.0] | [1.0-1.4] | [0.5-1.6] | [0.5-0.8] | [0.7-1.0] | [0.7-1.8] | [0.7-1.6] | [0.6-0.9] | |
| Bmal1 | 1.0 ± 0.3 | 0.1 ± 0.08 | 0.3 ± 0.1 | 1.0 ± 0.7 | 0.7 ± 0.3 | 0.4 ± 0.2 | 0.4 ± 0.2 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.9 ± 0.5 | 1.4 ± 0.5 | 0.1 ± 0.1 |
| 1.0 | 0.1 | 0.3 | 1.0 | 0.6 | 0.3 | 0.5 | 0.2 | 0.2 | 0.8 | 1.2 | 0.07 | |
| [0.8-1.3] | [0.06-0.2] | [0.1-0.4] | [0.4-1.6] | [0.4-0.8] | [0.2-0.5] | [0.1-0.6] | [0.07-0.4] | [0.08-0.4] | [0.6-1.0] | [0.9-1.9] | [0.03-0.1] | |
| Silt1 | 1.1 ± 0.5 | 1.2 ± 1.3 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.2 | 0.4 ± 0.1 | 1.3 ± 1.0 | 2.0 ± 1.4 | 0.2 ± 0.02 | 2.2 ± 2.5 | 0.8 ± 0.6 | 0.2 ± 0.1 |
| 1.0 | 0.6 | 0.2 | 0.3 | 0.3 | 0.4 | 1.1 | 2.1 | 0.2 | 1.0 | 0.5 | 0.2 | |
| [0.7-1.7] | [0.4-2.4] | [0.2-0.3] | [0.2-0.3] | [0.2-0.5] | [0.4-0.5] | [0.3-2.3] | [0.6-3.3] | [0.1-0.2] | [0.5-4.2] | [0.3-1.1] | [0.1-0.3] | |
| Ampk | 1.1 ± 0.3 | 0.9 ± 0.3 | 0.9 ± 0.5 | 2.8 ± 0.9 | 3.5 ± 1.4 | 1.2 ± 0.3 | 1.0 ± 0.5 | 1.5 ± 0.5 | 1.0 ± 0.5 | 1.0 ± 0.3 | 1.0 ± 0.3 | 1.1 ± 0.6 |
| 1.0 | 0.9 | 0.9 | 2.9 | 3.7 | 1.1 | 1.0 | 1.6 | 1.0 | 1.1 | 1.0 | 1.1 | |
| [0.9-1.2] | [0.6-1.2] | [0.5-1.4] | [1.9-3.4] | [1.8-5.1] | [1.0-1.4] | [0.6-1.4] | [1.0-1.8] | [0.3-1.4] | [0.8-1.3] | [0.8-1.3] | [0.5-1.5] | |
| Nampt | 1.0 ± 0.2 | 1.4 ± 0.8 | 2.3 ± 0.8 | 2.9 ± 0.7 | 2.6 ± 1.4 | 1.9 ± 0.4 | 2.6 ± 1.2 | 3.6 ± 1.9 | 4.0 ± 2.0 | 2.5 ± 1.4 | 3.0 ± 1.0 | 4.0 ± 3.8 |
| 1.0 | 1.3 | 2.1 | 3.1 | 2.5 | 2.0 | 2.4 | 4.6 | 3.6 | 1.9 | 3.1 | 2.3 | |
| [0.8-1.1] | [0.5-2.2] | [1.8-3.0] | [2.2-3.4] | [1.4-3.8] | [1.5-2.2] | [1.6-3.7] | [1.5-5.1] | [2.3-67] | [1.5-4.1] | [2.3-4.0] | [1.7-5.2] | |
| Pparg | 1.1 ± 0.4 | 1.4 ± 0.4 | 2.0 ± 0.5 | 3.6 ± 1.2 | 2.8 ± 0.7 | 5.4 ± 1.9 | 1.1 ± 0.7 | 1.5 ± 0.6 | 0.9 ± 0.3 | 1.5 ± 0.4 | 1.2 ± 0.3 | 0.8 ± 0.6 |
| 1.0 | 1.6 | 1.9 | 3.2 | 2.6 | 5.0 | 1.0 | 1.6 | 0.8 | 1.4 | 1.3 | 0.7 | |
| [0.8-1.4] | [1.0-1.7] | [1.7-2.3] | [3.0-4.8] | [2.1-3.6] | [5.4-6.9] | [0.7-1.7] | [1.0-2.0] | [0.7-1.3] | [1.1-1.8] | [0.9-1.4] | [0.3-1.2] | |
| Pgc1a | 1.1 ± 0.4 | 1.7 ± 1.0 | 1.1 ± 0.5 | 2.9 ± 0.9 | 2.6 ± 0.4 | 3.3 ± 1.0 | 1.4 ± 0.4 | 2.4 ± 1.1 | 0.8 ± 0.2 | 2.4 ± 0.8 | 1.3 ± 0.3 | 0.7 ± 0.3 |
| 1.0 | 1.4 | 0.9 | 3.1 | 2.5 | 3.4 | 1.5 | 3.0 | 0.7 | 2.1 | 1.3 | 0.6 | |
| [0.8-1.4] | [1.1-2.4] | [0.8-1.3] | [2.0-3.7] | [2.3-3.0] | [2.5-4.0] | [0.9-1.7] | [1.3-3.3] | [0.6-0.9] | [1.7-3.4] | [1.0-1.5] | [0.4-1.0] | |
| Ucp2 | 0.9 ± 0.2 | 1.4 ± 0.8 | 0.8 ± 0.2 | 1.6 ± 0.3 | 1.6 ± 0.5 | 1.8 ± 0.9 | 1.3 ± 0.6 | 2.5 ± 1.4 | 0.6 ± 0.2 | 1.9 ± 0.9 | 0.8 ± 0.2 | 0.5 ± 0.3 |
| 1.0 | 1.5 | 0.8 | 1.5 | 1.6 | 1.6 | 1.1 | 2.4 | 0.6 | 1.7 | 0.8 | 0.4 | |
| [0.6-1.1] | [0.7-2.1] | [0.6-1.0] | [1.4-1.8] | [1.1-2.2] | [1.0-2.2] | [0.8-1.9] | [1.2-3.7] | [0.3-0.7] | [1.5-2.3] | [0.7-1.0] | [0.3-0.7] | |
Data (2-ΔΔCt) are shown as mean ±; standard deviation, median and [interquartile interval]. CG: control group, DF: day-fed, RF-n: restricted night-fed, and RF-d: restricted day-fed. ZT3, ZT11 and ZT17: Zeitgeber times 3, 11 and 17, corresponding to 0900, 1700, and 2300h, respectively.