Supplementary Table 3. Relative expression of energy-sensing and metabolism-related genes in rat peri-epididymal adipose tissue on restricted feeding schedules.
| Gene | Peri-epididymal adipose tissue | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CG | DF | RF-n | RF-d | |||||||||
| ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | ZT3 | ZT11 | ZT17 | |
| Clock | 1.0 ± 0.5 | 0.8 ± 0.3 | 1.0 ± 0.2 | 0.5 ± 0.1 | 0.5 ± 0.2 | 0.4 ± 0.1 | 1.0 ± 0.3 | 0.3 ± 0.1 | 0.5 ± 0.1 | 0.8 ± 0.2 | 0.8 ± 0.6 | 0.4 ± 0.1 |
| 1.0 | 0.8 | 0.9 | 0.5 | 0.5 | 0.4 | 0.9 | 0.3 | 0.5 | 0.8 | 0.6 | 0.4 | |
| [0.5-1.4] | [0.6-0.9] | [0.8 ± 1.2] | [0.4-0.7] | [0.4-0.7] | [0.4-0.5] | [0.8-1.3] | [0.2-0.4] | [0.4-0.6] | [0.6-1.0] | [0.4-1.6] | [0.3-0.6] | |
| Bmal1 | 1.0 ± 0.3 | 0.06 ± 0.04 | 0.2 ± 0.1 | 0.2 ± 0.07 | 0.2 ± 0.08 | 0.1 ± 0.06 | 0.3 ± 0.1 | 0.04 ± 0.02 | 0.4 ± 0.1 | 0.2 ± 0.07 | 1.0 ± 0.3 | 0.2 ± 0.1 |
| 1.0 | 0.07 | 0.1 | 0.2 | 0.2 | 0.1 | 0.3 | 0.04 | 0.4 | 0.2 | 1.0 | 0.1 | |
| [0.7-1.2] | [0.03-0.1] | [0.1-0.3] | [0.1-0.2] | [0.1-0.3] | [0.07-0.2] | [0.2-0.4] | [0.02-0.05] | [0.3-0.5] | [0.1-0.2] | [0.8-1.1] | [0.07-0.3] | |
| Silt1 | 1.6 ± 1.1 | 2.7 ± 2.6 | 0.9 ± 0.4 | 0.8 ± 0.3 | 0.8 ± 0.4 | 0.4 ± 0.04 | 1.3 ± 0.6 | 1.6 ± 0.8 | 0.5 ± 0.2 | 1.9 ± 0.6 | 0.7 ± 0.2 | 0.5 ± 0.3 |
| 1.0 | 1.7 | 0.9 | 0.8 | 0.7 | 0.4 | 1.1 | 1.1 | 0.4 | 2.1 | 0.8 | 0.4 | |
| [0.9-2.8] | [0.8-4.6] | [0.5-1.3] | [0.5-1.1] | [0.5-1.0] | [0.3-0.4] | [0.8-1.9] | [1.0-2.4] | [0.4-0.7] | [1.4-2.2] | [0.5-0.8] | [0.3-0.8] | |
| Ampk | 1.4 ± 1.1 | 2.2 ± 2.6 | 0.9 ± 0.3 | 0.9 ± 0.4 | 1.8 ± 0.5 | 0.5 ± 0.1 | 1.2 ± 1.4 | 0.8 ± 0.9 | 0.7 ± 0.3 | 0.7 ± 0.4 | 0.6 ± 0.2 | 0.6 ± 0.2 |
| 1.0 | 1.3 | 0.9 | 0.9 | 1.6 | 0.5 | 0.6 | 0.5 | 0.7 | 0.6 | 0.6 | 0.6 | |
| [0.7-2.0] | [0.7-3.4] | [0.5-1.2] | [0.5-1.3] | [1.4-2.3] | [0.4-0.6] | [0.5-1.6] | [0.4-1.5] | [0.6-0.7] | [0.4-1.1] | [0.5-0.8] | [0.5-0.8] | |
| Nampt | 1.0 ± 0.4 | 3.7 ± 4.0 | 5.1 ± 2.6 | 1.5 ± 0.5 | 0.8 ± 0.3 | 0.9 ± 0.1 | 2.0 ± 1.5 | 1.9 ± 0.7 | 2.0 ± 0.4 | 3.3 ± 1.8 | 2.1 ± 0.9 | 1.6 ± 0.8 |
| 1.0 | 2.3 | 4.2 | 1.3 | 1.0 | 0.9 | 1.5 | 1.6 | 2.1 | 2.4 | 2.2 | 1.4 | |
| [0.8-1.4] | [1.7-4.7] | [3.2-8.1] | [1.1 -2.1] | [0.5-1.0] | [0.8-1.1] | [1.1-2.6] | [1.4-2.5] | [1.7-2.2] | [1.4-5.1] | [1.5-2.4] | [1.2-1.9] | |
| Pparg | 0.9 ± 0.3 | 1.8 ± 0.8 | 0.9 ± 0.3 | 1.2 ± 0.6 | 0.8 ± 0.3 | 0.5 ± 0.1 | 1.8 ± 1.6 | 1.9 ± 0.5 | 0.5 ± 0.3 | 1.9 ± 0.8 | 1.1 ± 0.6 | 0.7 ± 0.4 |
| 1.0 | 1.9 | 0.9 | 1.1 | 0.8 | 0.5 | 1.7 | 2.2 | 0.6 | 1.6 | 1.0 | 0.5 | |
| [0.5-1.3] | [1.0-2.6] | [0.8-1.1] | [0.7-1.8] | [0.6-1.0] | [0.4-0.6] | [0.6-2.5] | [1.5-2.3] | [0.2-0.9] | [1.4-2.3] | [0.6-1.7] | [0.4-1.0] | |
| Pgc1a | 1.1 ± 0.7 | 1.3 ± 1.0 | 0.5 ± 0.2 | 0.7 ± 0.2 | 1.2 ± 0.4 | 0.5 ± 0.2 | 1.8 ± 1.4 | 1.4 ± 0.8 | 1.5 ± 2.4 | 2.3 ± 2.3 | 1.9 ± 1.6 | 0.5 ± 0.2 |
| 1.0 | 1.2 | 0.6 | 0.7 | 1.3 | 0.5 | 1.2 | 1.5 | 0.7 | 2.1 | 1.2 | 0.4 | |
| [0.8-1.6] | [0.4-2.0] | [0.4-0.6] | [0.6-0.9] | [0.9-1.5] | [0.3-0.6] | [0.8-3.0] | [0.7-2.1] | [0.4-1.0] | [0.7-2.5] | [0.9-2.2] | [0.3-0.8] | |
| Ucp2 | 1.1 ± 0.5 | 1.3 ± 0.6 | 0.1 ± 0.1 | 0.8 ± 0.4 | 0.7 ± 0.3 | 0.2 ± 0.1 | 1.1 ± 1.0 | 0.7 ± 0.2 | 0.4 ± 0.6 | 1.0 ± 0.4 | 0.6 ± 0.4 | 0.1 ± 0.1 |
| 1.0 | 1.1 | 0.04 | 0.7 | 0.8 | 0.2 | 0.6 | 0.8 | 0.2 | 1.0 | 0.4 | 0.05 | |
| [0.6-1.6] | [0.8-1.7] | [0.02-0.2] | [0.5-1.2] | [0.5-1.0] | [0.1-0.3] | [0.4-2.3] | [0.5-0.9] | [0.1-0.9] | [0.6-1.4] | [0.3-0.7] | [0.02-0.2] | |
Data (2-ΔΔCt) are shown as mean ± standard deviation, median and [interquartile interval]. CG: control group, DF: day-fed, RF-n: restricted night-fed, and RF-d: restricted day-fed. ZT3, ZT11 and ZT17: Zeitgeber times 3, 11 and 17, corresponding to 0900, 1700, and 2300h, respectively.