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. Author manuscript; available in PMC: 2024 Sep 25.
Published in final edited form as: Angew Chem Int Ed Engl. 2023 Aug 18;62(39):e202308650. doi: 10.1002/anie.202308650

Figure 5.

Figure 5.

Hydroxyl radical footprinting of 5’-32P-radiolabeled RNA 1 with CHF-2. Lane 1, 5’-32P-radiolabeled RNA 1; lane 2, NaHCO3 hydrolysis ladder; lane 3, RNase T1 standard; Lane 4, hydroxyl radical (·OH), lane 5-20, 2-fold serial dilution of 1 mM to 0.03 μM CHF-2. Footprint signatures are labeled in brackets (i) to (iv). Footprints corresponding to RNA 1 sequence are denoted with Roman numerals and red dashed boxes. Overlay of CHF-2 corresponds to the binding site for RNA 1.