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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Anal Bioanal Chem. 2023 Jul 20;415(23):5605–5617. doi: 10.1007/s00216-023-04856-8

Fig. 3.

Fig. 3

a. rRT-LAMP assay of 5 × 106 to 50 GEs MAYV RNA per reaction. All plots are an average of three replicates. b. Calibration curve between threshold time and MAYV concentration. c. RT-LAMP endpoint detection of 15, 10, and 5 GEs of MAYV RNA. The endpoint detection was carried out after 30-min RT-LAMP reactions. NTC stands for no-template control. d. No cross-reactivity was observed for MAYV-targeted RT-LAMP assay when it was carried out against ZIKV, CHIKV, DENV-1, DENV-4, and a mixture containing these four viruses.