KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-FLAG, rabbit sourced | Sigma | Cat#F7425 |
| anti-LAMP-1, rabbit sourced | Abcam | Cat#ab24170 |
| anti-LAMP-2, rabbit sourced | Abcam | Cat#ab199946 |
| Anti-FLAG, mouse sourced | Sigma | Cat#F3165 |
| Anti-TMEM175, rabbit sourced | Proteintech | Cat#19925-1-AP |
| anti-Cathepsin B, rabbit sourced | Abcam | Cat#ab125067 |
| anti-β actin, rabbit sourced | Sigma | Cat#A2066 |
| anti-LAMP1, mouse sourced | Abcam | Cat#ab25630 |
| anti-LAMP2, mouse sourced | Abcam | Cat#ab25631 |
| Bacterial and Virus Strains | ||
| DH5a competent E.coli | Thermo Fisher | Cat# 18265017 |
| E. coli DH10bac | Thermo Fisher | Cat# 10361012 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| n-Dodecyl-β-D-Maltopyranoside | Anatrace | Cat#D310 |
| Dithiothreitol | Thermo | Cat#R0861 |
| Iodoacetamide | Sigma | Cat#I6125 |
| Acetonitrile | Fisher | Cat#75-05-8 |
| Trypsin Gold | Promega | Cat#V5280 |
| 6-Aminohexanoic acid | Sigma | Cat#07260 |
| Sulfo-NHS-SS-Biotin | Thermo | Cat#21331 |
| GDN | Anatrace | Cat#GDN101 |
| Endo Hf | NEB | Cat#P0703S |
| Carbonyl cyanide 3-chlorophenylhydrazone | Sigma | Cat#C2759 |
| ACMA (9-Amino-6-Chloro-2-Methoxyacridine) | Thermo | Cat#A1324 |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (POPG) | Avanti | Cat#840457C |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) | Avanti | Cat#850757P |
| n-Decyl-β-D-Maltoside | Anatrace | Cat#D322LA |
| Bio-Beads SM-2 Resin | Bio-rad | Cat#1523920 |
| Valinomycin | Invitrogen | Cat#V1644 |
| BS3 (bis(sulfosuccinimidyl)suberate) | Thermo | Cat#21580 |
| LysoTracker™ Red DND-99 | Invitrogen | Cat#L7528 |
| Oregon Green™ 488 dextran; 10,000 MW, Anionic | Invitrogen | Cat#D7170 |
| Nigericin | Invitrogen | Cat#N1495 |
| DQ™ Red BSA | Invitrogen | Cat#D12051 |
| Magic Red® Cathepsin-B Assay Kit | Immunochemistry | Cat#937 |
| FLAG peptide | Sigma | Cat#F3290 |
| Deposited Data | ||
| TMEM175-full length LAMP-1 density map | This study | EMD: EMD-29553 |
| TMEM175-full length LAMP-1 atomic model | This study | PDB: 8FY5 |
| TMEM175-TM LAMP-1 density map | This study | EMD: EMD-29572 |
| TMEM175-TM LAMP-1 atomic model | This study | PDB: 8FYF |
| The raw data of quantitative proteomics of TMEM175 affinity enrichment | This study | http://dx.doi.org/doi:10.25345/C5RN30J1P |
| The raw data of crosslinking proteomics of TMEM175-LAMP1 complex | This study | http://dx.doi.org/doi:10.25345/C5CC0V39R |
| The Mendeley dataset of raw data of gels, blots and imagings | This study | http://dx.doi.org/doi:10.17632/6d8w9vv5bv.3 |
| Experimental Models: Cell Lines | ||
| HEK293 | ATCC | ATCC#CRL-1573 |
| SH-SY5Y | ATCC | ATCC#CRL-2266 |
| Sf9 | ATCC | ATCC#CRL-1711 |
| FreeStyle™ 293-F | ThermoFisher | Cat#R79007 |
| HAP1 | HorizonDiscovery | Cat#HorizonDiscovery |
| HAP1 TMEM175 knockout | HorizonDiscovery | Cat#HZGHC005593c004 |
| Recombinant DNA | ||
| pCMV6-TMEM175-Myc-FLAG | Origene | Cat#RC201422 |
| pCMV6-LAMP1-Myc-FLAG | Origene | Cat#RC219208 |
| pCMV3-FLAG-LAMP1 | SinoBiological | Cat#HG11215-NF |
| pCMV3-FLAG-LAMP2 | SinoBiological | Cat#HG13555-NF |
| pEZT-BM | Addgene | Plasmid#74099 |
| pCMV6-LAMP1-GFP | Origene | Cat#RC100016 |
| pCMV6-LAMP2-GFP | Origene | Cat#RG200456 |
| Software and Algorithms | ||
| Maxquant | Maxquant 2.1.4 | https://www.maxquant.org |
| Mascot | Mascot 2.7.0 | http://www.matrixscience.com/ |
| RELION | Scheres, 2012 | https://www3.mrc-lmb.cam.ac.uk/relion |
| UCSF ChimeraX | Pettersen et al., 2021 | https://www.cgl.ucsf.edu/chimerax/ |
| PHENIX | Adams et al., 2010 | https://www.phenix-online.org |
| COOT | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PyMOL | Molecular Graphics System, Version 2.5.2, Schrödinger | https://pymol.org/2/ |
| Fiji | Schindelin et al., 2012 | https://imagej.net/software/fiji/ |
| Other | ||
| R1.2/1.3 200-mesh or 300-mesh gold holey carbon grid | Quantifoil | Cat#4220G-CF/4230G-CF |