KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| CD3-FITC | BD Biosciences | Cat#556611; RRID:AB_396484 Clone-SP34 |
| CD20-BV510 | BioLegend | Cat#302340; RRID:AB_2561941 Clone-2H7 |
| CD56-BV711 | BioLegend | Cat#318336; RRID:AB_2562417 Clone-HCD56 |
| CD16-PB | BioLegend | Cat#302021; RRID:AB_492978 Clone-3G8 |
| CD14-AF700 | BioLegend | Cat#301822; RRID:AB_493747 Clone-M5E2 |
| HLA-DR-APC-Cy7 | BioLegend | Cat#307618; RRID:AB_493586 Clone-L243 |
| CD11c-APC | BioLegend | Cat#301614; RRID:AB_493023 Clone-3.9 |
| CD123-PCP-Cy5.5 | BioLegend | Cat#306016; RRID:AB_2264693 Clone-6H6 |
| CD86-BV605 | BioLegend | Cat#305430; RRID:AB_2563824 Clone-IT2.2 |
| CD4-FITC | BioLegend | Cat#300538; RRID:AB_2562052 Clone-RPA-T4 |
| CD4-PE | BioLegend | Cat#317410; RRID:AB_571955 Clone-OKT4 |
| CD8b-ECD | Beckman Coulter | Cat#6607123; RRID:AB_1575983 Clone-2ST8.5H7 |
| CD45RA-PerCP-Cy5.5 | Thermo Fisher Scientific | Cat#45-0458-42; RRID:AB_10718536 Clone-HI100 |
| CCR7-PE-Cy7 | BioLegend | Cat#353226; RRID:AB_11126145 Clone-GO43H7 |
| CD69-PE | BioLegend | Cat#310906; RRID:AB_314841 Clone-FN50 |
| CD103-BV605 | BioLegend | Cat#350218; RRID:AB_2564283 Clone-BER Act1 |
| CTLA4-PeDazzle594 | BioLegend | Cat#369616; RRID:AB_2632878 Clone-BNI3 |
| CD25-APC-Cy7 | BioLegend | Cat#302614; RRID:AB_314284 Clone-BC96 |
| FOXP3-A488 | BioLegend | Cat#320106; RRID:AB_439752 Clone-206D |
| CD49a-PE-Cy7 | BioLegend | Cat#328312; RRID:AB_2566272 Clone-TS2/7 |
| CD39-BV510 | BioLegend | Cat#328219; RRID:AB_2563265 Clone-A1 |
| ITGB2 (CD11a/CD18)-PCP-Cy5.5 | BioLegend | Cat#302120; RRID:AB_2565587 Clone-TS1/18 |
| KIR2DL4 (CD158d)-APC | BioLegend | Cat#347008; RRID:AB_2130691 Clone-mAb33 |
| KIR2DL1 (CD158a)-APC-Cy7 | Novus Biologicals | Cat#NB100-63267APCCY7; RRID:AB_2941073 Clone-NKVFS1 |
| KIR2DL2/3 (CD158b)-PE | BD Biosciences | Cat#559785; RRID:AB_397326 Clone-CH-L |
| CD160-AF700 | Novus Biologicals | Cat#NBP2-90074AF700; RRID:AB_2941072 Clone-275 |
| NKG2A-PE-Dazzle594 | BioLegend | Cat#375121; RRID:AB_2888869 Clone-S19004C |
| NKG2C-PE | BioLegend | Cat#375003; RRID:AB_2888871 Clone-S19005E |
| CD9-PE-Cy7 | BioLegend | Cat#312116; RRID:AB_2728256 Clone-HI9a |
| CD68-APC | BioLegend | Cat#333810; RRID:AB_2275735 Clone-Y1/82A |
| TLR4-BV711 | BD Biosciences | Cat#564404; RRID:AB_2738794 Clone-tf901 |
| CD163-APC/Fire750 | BioLegend | Cat#333634; RRID:AB_2734333 Clone-GHI/61 |
| CD206-BV510 | BioLegend | Cat#321138; RRID:AB_2721530 Clone-15-2 |
| CD209-FITC | BioLegend | Cat#330104; RRID:AB_1134048 Clone-9e9a8 |
| TREM1-AF405 | R&D Systems | Cat#FAB12781V; RRID:AB_2941074 Clone-888111 |
| TREM2-PerCP | R&D Systems | Cat#FAB17291C; RRID:AB_2941075 Clone-237920 |
| CD64-BV711 | BioLegend | Cat#305042; RRID:AB_2800778 Clone-10.1 |
| CD64-APC | BioLegend | Cat#305014; RRID:AB_1595428 Clone-10.1 |
| GLUT1-AF488 | R&D Systems | Cat#FAB1418G; RRID:AB_2941076 Clone-202915 |
| FOLR2-PE | BioLegend | Cat#391704; RRID:AB_2721336 Clone-94b/folr2 |
| CCR2-BV605 | BioLegend | Cat#357213; RRID:AB_2562702 Clone-K036C2 |
| CD19-PE | BioLegend | Cat#302208; RRID:AB_314238 Clone-HIB19 |
| S100A9-APC | Invitrogen | Cat#MA5-28129; RRID:AB_2745112 Clone-10.1 |
| CD107a-FITC | BD Biosciences | Cat#555800; RRID:AB_396134 Clone-H4A3 |
| PD-1-BV510 | BioLegend | Cat#329932; RRID:AB_2562256 Clone-Eh12.2h7 |
| CD45-FITC | BioLegend | Cat#304038; RRID:AB_2562050 Clone-HI30 |
| TNFα-APC | BioLegend | Cat#502912; RRID:AB_315264 Clone-Mab11 |
| IL-6-FITC | BD Biosciences | Cat#554696; RRID:AB_395514 Clone-MQ2-6A3 |
| IL-1β-PB | BioLegend | Cat#511710; RRID:AB_2124350 Clone-H1b-98 |
| IFNγ-APC | Invitrogen | Cat#17-7319-82; RRID:AB_469506 Clone-4S.B3 |
| IFNγ-PE-Cy7 | eBioscience | Cat#502527; RRID:AB_1626154 Clone-4S.B3 |
| IL-2-AF700 | BioLegend | Cat#500320; RRID:AB_528929 Clone-MQ-17H12 |
| IL-4-APC | Tonbo Biosciences | Cat#50-210-2786; RRID:AB_2941077 Clone-MP425D2 |
| IL-17-FITC | Invitrogen | Cat#11-717942; RRID:AB_10805390 Clone-Ebio64dec17 |
| TGF-β-PerCP-Cy5.5 | BioLegend | Cat#141410; RRID:AB_2561592 Clone-TW7-16B4 |
| MIP-1β-PE | BD Biosciences | Cat#550078; RRID:AB_393549 Clone-D21-1351 |
| GZMB-AF700 | BD Biosciences | Cat#560213; RRID:AB_1645453 Clone-GB11 |
| NF-κB p50-AF488 | Luminex | Cat#4700-1674; RRID:AB_2941078 |
| 7-Amino-Actinomycin D (7-AAD) | Luminex | Cat#400-0290; RRID:AB_2941079 |
| NF-κB p65 (pS529)-AF647 | BD Biosciences | Cat#558422; RRID:AB_647136 Clone-K10-895.12.50 |
| IκBa-PE | eBioscience | Cat#12-903642; RRID:AB_2572683 Clone-MFRDTRK |
| Phospho-p38 MAPK-APC | eBiosceince | Cat#17-9078-42; RRID:AB_2573290 Clone-4NIT4KK |
| CD3-PE | BD Biosciences | Cat#556612; RRID:AB_396485 Clone-SP34 |
| TotalSeq-C0072-CD4 | BioLegend | Cat#300567; RRID:AB_2800725 Clone-RPA-T4 |
| TotalSeq-C0046-CD8 | BioLegend | Cat#344753; RRID:AB_2800922 Clone-SK1 |
| TotalSeq-C0148-CCR7 | BioLegend | Cat#353251; RRID:AB_2800943 Clone-G043H7 |
| TotalSeq-C0063-CD45RA | BioLegend | Cat#304163; RRID:AB_2800764 Clone-HI100 |
| TotalSeq-C0146-CD69 | BioLegend | Cat#310951; RRID:AB_2800810 Clone-FN50 |
| TotalSeq-C0145-CD103 | BioLegend | Cat#350233; RRID:AB_2800933 Clone-Ber-ACT8 |
| TotalSeq-C0088-PD-1 | BioLegend | Cat#329963; RRID:AB_2800862 Clone-EH12.2H7 |
| TotalSeq-C0085-CD25 | BioLegend | Cat#302649; RRID:AB_2800745 Clone-BC96 |
| TotalSeq-C0251 | BioLegend | Cat#394661; RRID:AB_2801031 Clone-LNH-94, 2M2 |
| TotalSeq-C0254 | BioLegend | Cat#394667; RRID:AB_2801034 Clone-LNH-94, 2M2 |
| TotalSeq-C0256 | BioLegend | Cat#394671; RRID:AB_2820042 Clone-LNH-94, 2M2 |
| TotalSeq-C0260 | BioLegend | Cat#394679; RRID:AB_2820046 Clone-LNH-94, 2M2 |
|
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| Biological Samples | ||
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| Fetal Bovine Serum, USDA Certified, Heat Inactivated | Omega Scientific | Cat#FB-02 |
| FetalPlex™ Animal Serum Complex | GeminiBioProducts | Cat#100-602 |
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| Chemicals, Peptides, and Recombinant Proteins | ||
|
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| RPMI 1640 | VWR | Cat#45000-396 |
| Hanks Balanced Salt Solution (HBSS) | VWR | Cat#21-023-CV |
| Penicillin Streptomycin | GeminiBio | Cat#400109 |
| L-Glutamine | GeminiBio | Cat#400106 |
| N-2-hydroxyethylpiperazine-N′2-ethanesulfonic acid HEPES | ThermoFisher | Cat#15630080 |
| Collagenase | Sigma | Cat# C9722 Source: Clostridium histolyticum |
| Ammonium chloride - NH4Cl (RBC Lysis Buffer) | Sigma | Cat#A9434 |
| Sodium hydrogen carbonate - CHNaO3 (RBC Lysis Buffer) | Fisher | Cat#AAA170050E |
| EDTA (RBC Lysis Buffer) | Invitrogen | Cat#AM9260G |
| Percoll Density Gradient | Neta Scientific | Cat# 17-0891-01 |
| Ficoll-Paque | GE Healthcare | Cat# 17144003 |
| Dimethyl sulfoxide (DMSO) | Sigma | Cat# D2650-100ML |
| FOXP3 Fix/Perm Buffer Set | BioLegend | Cat#421403 |
| Ghost Dye 510 | TONBO Biosciences | Cat#13-0870-T 100 |
| Ghost Dye 540 | TONBO Biosciences | Cat#13-0879 |
| SYTOX Blue Dead Cell Stain | ThermoFisher Scientific | Cat#S34857 |
| Human TruStain FcX | BioLegend | Cat#422302 |
| Human True-Stain Monocyte Blocker | BioLegend | Cat#426103 |
| Lipopolysaccharide (LPS) TLR4 ligand E. coli 055:B5 | Invivogen | Cat#tlrl-b5lps |
| Pam3CSK4 TLR1/2 agonist | Invivogen | Cat#tlrl-pm2s-1 |
| FSL-1 TLR2/6 agonist | Sigma | Cat#SML1420 |
| E. coli | Migula. Castellani and Chalmers ATCC 11775 | Cat#1132342 |
| Phorbol myristate acetate-NF-κB Activator (PMA) | Invivogen | Cat#tlrl-pma |
| Ionomycin | Invivogen | Cat#inh-ion |
| Brefeldin A (BFA) | BioLegend | Ca#420601 |
| Fixation buffer | BioLegend | Cat#420801 |
| Amnis NF-κB translocation kit | Luminex Corporation | Cat #ACS10000 |
| Permeabilization wash buffer | BioLegend | Cat#421002 |
| Bovine Serum Albumin (BSA), Fraction V— Molecular Biology Grade |
GeminiBio | Cat#700-106P |
| Cytofix fixation buffer | BD Biosciences | Cat#554655 |
| BD Perm/Wash buffer | BD Biosciences | Cat#554723 |
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| Critical Commercial Assays | ||
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| BODIPY 493/503 | ThermoFisher Scientific | Cat#D3922 |
| pHrodo Deep Red E. coli BioParticle Conjugates | ThermoFisher Scientific | Cat#P35360 |
| CellROX Deep Red Flow Cytometry Assay Kit | ThermoFisher Scientific | Cat#C10491 |
| 2-NBDG | ThermoFisher Scientific | Cat#N13195 |
| MitoTracker Red CMXRos | ThermoFisher Scientific | Cat#M7512 |
| Seahorse XF Cell Energy Phenotype kit | Agilent Technologies | Cat#103592-100 |
| Human Premixed 29-plex Magnetic Luminex Assay | R&D Systems | Cat#Custom-LxSA-H-29 Lot#C0003514 |
| TotalSeq B Hashtag Oligos 1-10 | BioLegend | 384631-392649 |
| Chromium Single Cell 3’ Reagent Kits v3 | 10X Genomics | PN-1000075 |
| 3′ Feature Barcode Kit | 10X Genomics | PN-1000262 |
| Chromium Single Cell 5’ Reagent Kits V2 | 10X Genomics | PN-1000263 |
| 5′ Feature Barcode Kit | 10X Genomics | PN-1000256 |
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| Deposited Data | ||
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| 3′ Single Cell GEX data - Decidua | This paper | NCBI Sequence Read Archive: PRJNA817521 |
| 5′ Single Cell TCR data - Decidua | This paper | NCBI Sequence Read Archive: PRJNA817521 |
| 5′ Single Cell TCR data - Maternal PBMC | This paper | NCBI Sequence Read Archive: PRJNA817521 |
| 3′ Single Cell GEX data -Villous | This paper | NCBI Sequence Read Archive:PRJNA946160 |
| 3′ Single Cell GEX data - UCBMC | Sureshchandra et al. 202166 10.3389/fimmu.2021.617592 |
NCBI Sequence Read Archive:PRJNA690128 |
| 3′ Single Cell GEX data - Maternal PBMC | This paper and Sureshchandra etal. 202166 10.1016/j.isci.2021.102690 |
NCBI Sequence Read Archive:PRJNA887020 |
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| Software and Algorithms | ||
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| Prism | GraphPad | Version#8 |
| Immunarch | R package | Version#0.6.7 |
| Metascape | www.metascape.org | N/A |
| Attune NxT Flow Cytometer Software | ThermoFisher Scientific | Version#2.5 |
| FlowJo | TreeStar | Version#10.5 |
| Ideas Analysis Software | Luminex Corporation | Version#6.1 |
| Seahorse Wave | Agilent Technologies | Version#2.6.1 |
| xPONENT® Software for Luminex Instruments | Luminex Corporation | Version#4.3 |
| Cell Ranger Single-Cell Software Suite | 10X Genomics | Version 6.0.2 |
| Seurat | R package | Version 3.1.5 |
| Monocle | R package | Version 2.8.0 |