A conserved pressure-driven mechanism for regulating cytosolic osmolarity

. Author manuscript; available in PMC: 2024 Aug 21.

Published in final edited form as: Curr Biol. 2023 Jul 20;33(16):3325–3337.e5. doi: 10.1016/j.cub.2023.06.061

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Low-melt agarose	Affymetrix	Cat#32830 25 GM
Sorbitol	Sigma Aldrich	Cat#S1876-500G
Latrunculin B	Abcam	Cat#ab144291
CK-666	Calbiochem/Sigma Aldrich	Cat#182515
CK-689	Calbiochem/Sigma Aldrich	Cat#182517
DMSO	Sigma Aldrich	Cat#D2650-5x5ML
Nocodazole	Sigma Aldrich	Cat#M1404
Bafilomycin A1	Sigma Aldrich	Cat#19-148
FM^™ 4-64 Dye	Invitrogen	Cat#T13320
Alexa Fluor^™ 488 Phalloidin	Thermo Fisher	Cat#A12379
Deposited Data
Pfam-A database (v35.0)	⁹⁵	RRID:SCR_004726
JGI genome portal (N. gruberi proteins Naegr1_best_proteins.fasta)	⁸⁶	RRID:SCR_002383
Experimental Models: Cell Lines
Naegleria gruberi strain NEG-M	Chandler Fulton	ATCC30224
Dictyostelium discoideum strain Ax2-ME (cloned and expanded from strain AX2-RRK)	Dictybase stock center	DBS0235521
Recombinant DNA
RFP-LimE plasmid	Dictybase.org	475
Software and Algorithms
NIS Elements with Advanced Research Package	Nikon Instruments	RRID:SCR_014329
Fiji	⁹⁴	RRID:SCR_002285
GraphPad Prism v9	GraphPad	RRID:SCR_002798
Custom code	This work	Zenodo (doi: 10.5281/zenodo.8034763)