Table 1.
Significantly changing prohormone-derived pituitary peptides identified from the Oct. (Tb = 21-35 °C) vs. Aug. (Active) comparison.
| Peptide Sequencea | Peptide Nameb | Gene | FC(log2)c |
|---|---|---|---|
| EESEESEEGTTSEVT | CHGB | 2.03 | |
| GEAYHHVPESQRDKA | CHGB | 1.49 | |
| EEASLQDRQYASHHT | CHGB | 1.41 | |
| GEAYHHVPESQRD | CHGB | 1.23 | |
| LDQLLHY | CHGB | 1.22 | |
| EEASLQDRQYASHHTTE | CHGB | 0.56 | |
| ELDQLLHY | CHGB | 1.17 | |
| Q(−17.03)KIAEKFSQ | CHGB | 1.20 | |
| SPQLEDE | PENK | 0.99 | |
| YGGFM(+15.99)RF | Met-enkephalin-Arg-Phe | PENK | 0.93 |
| MYEENSRDNPF | SCG2 | 0.79 | |
| FPTLGGSQDKSLHN | SCG3 | 0.42 | |
| TLGGSQDKSLHN | SCG3 | 1.64 | |
| SANSNPAM(+15.99)APRE | Somatostatin-28(1-12) | SST | 2.14 |
| SANSNPAMAPRE | Somatostatin-28(1-12) | SST | 1.07 |
| Peptide Sequencea | Peptide Nameb | Gene | FC(log2)c |
| AGAPEPAEPMELAKPSA | Copeptin(25-41) | AVP | −4.12 |
| LAGAPEPA | Copeptin(24-31) | AVP | −5.23 |
| LGVLFNPYYDPLQWKSSRFE | CHGB | −1.28 | |
| Q(−17.03)YDRVAELDQLLHY | BAM-1745 | CHGB | −1.85 |
| AADQDLGPEAPPEGVLGA | PEN-18 | PCSK1N | −0.97 |
| AADQDLGPEAPPEGVLGAL | PEN-19 | PCSK1N | −1.86 |
| LENPQPQAPARRLLPP | BigLEN | PCSK1N | −1.02 |
| VKMALQQEGFD | PCSK2 | −1.75 | |
| DDGPYRMEHFRWGSPPKD | β-MSH | POMC | −2.11 |
| KYVMGHFRWD | Lys-γ-MSH(1-10) | POMC | −1.62 |
| KYVMGHFRWDRF(−.98) | Lys-γ-MSH | POMC | −2.33 |
| RPVKVYPNGAEDES(+79.97)AESFPLEF | CLIP | POMC | −2.53 |
| RPVKVYPNGAEDESAESFPLEF | CLIP | POMC | −1.97 |
| S(+42.01)YSMEHFRWGKPV(−.98) | α-MSH | POMC | −2.32 |
| SYSMEHFRWGKPV(−.98) | Desacetyl-α-MSH | POMC | −1.67 |
| Y(+42.01)GGFM(+15.99)TSEKSQTPLVTLFKNAIIKNAH | Ac-β-endorphin(1-27) | POMC | −3.27 |
| Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNA | Ac-β-endorphin(1-26) | POMC | −3.98 |
| Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAH | Ac-β-endorphin(1-27) | POMC | −4.00 |
| Y(+42.01)GGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ | Ac-β-endorphin(1-31) | POMC | −2.71 |
| YGGFMTSEKSQTPLVTLFKNAIIKNA | β-endorphin(1-26) | POMC | −1.22 |
| YGGFMTSEKSQTPLVTLFKNAIIKNAH | β-endorphin(1-27) | POMC | −1.32 |
| SVNPYLQGQRLDNVV | SCG5 | −0.73 |
a
Post-translational modifications are indicated by mass change observed by mass spectrometry. Amidation = (−.98), oxidation = (+15.99), pyroglutamylation = (−17.03), acetylation = (+42.01), phosphorylation = (+79.97).
b
Peptide common names inferred from analogy to well-annotated rat sequence.
c
Fold-change values are given for Oct./Aug. comparison. A positive value indicates the peptide is higher abundance in Oct.