. Author manuscript; available in PMC: 2024 Oct 1.

Published in final edited form as: Toxicol Appl Pharmacol. 2023 Aug 20;476:116659. doi: 10.1016/j.taap.2023.116659

Table 1.

Endpoint classes used in SYSTEMETRIC^® Cell Health Screen and the ZBEscreen^™.

Classes of endpoints used in in vitro (SYSTEMETRIC^®) and in vivo (zebrafish) systems

Endpoint Class	Zebrafish Endpoints	SYSTEMETRIC^® Endpoints
Mortality	Mortality (24 hpf, 120 hpf)
Morphology	Craniofacial malformation Curved/bent axis Yolk/heart edema Muscular malformation Lateral trunk malformation Brain malformation or necrosis Hyper/hypo pigmentation Notochord distortion Abnormal touch response
Behavior	Embryo photomotor response (EPR: 24 hpf) Larval photomotor response (LPR: 120 hpf)
Cytotoxicity		Cell morphology Cell membrane integrity ROS superoxide Glutathione depletion Nuclear membrane integrity 1 Cell cycle Nuclear membrane integrity 2 Mitochondrial membrane potential CHI probability