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. Author manuscript; available in PMC: 2024 Sep 20.
Published in final edited form as: Neuron. 2023 Jul 13;111(18):2863–2880.e6. doi: 10.1016/j.neuron.2023.06.009

Figure 5: Bulk transcriptome and Nkx2.1-IN specific translatome is altered in P15 Fmr1 KO mice.

Figure 5:

A. Experimental design for transcriptomic analysis at P15.

B. Cartoon of Ribotag approach for RNA-seq in S1 at P15.

C. Volcano plot of differentially expressed (DE) genes in the bulk RNA of Fmr1 KO-mCherry mice (n=8) compared to WT-mCherry (n=7).

D. Top 10 ‘GO terms’ of the bulk transcriptome in Fmr1 KO-mCherry mice using the biological process package (see Methods).

E. Number of DE genes in Fmr1 KO-mCherry mice within the bulk transcriptome dataset that are known targets of FMRP, based on previously published database (see Methods). Heatmaps represent the changes of expression as Z-scores among the top 25 upregulated and downregulated genes.

F. Volcano plot of DE genes in the Nkx2.1-IN-specific translatome of Fmr1 KO-mCherry mice (n=8) compared to WT-mCherry control (n=3).

G. Top 10 ‘GO terms’ in Nkx2.1-IN-specific translatome in Fmr1 KO-mCherry mice.

H. Number of DE genes in Fmr1 KO-mCherry mice within the Nkx2.1-specific translatome dataset that are known targets of FMRP (shown as in panel E).