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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: Vaccine. 2023 Jul 1;41(34):4967–4977. doi: 10.1016/j.vaccine.2023.06.052

Fig. 2. (A) 1H NMR of purified Sf2a OSP and Sf3a OSP antigens used in conjugation; (B) 13C NMR of same.

Fig. 2.

The signals of OSP predominate; however, some signature peaks, such as the two peaks in 13C NMR between 53 and 54 ppm representing C-2 of the two amino sugars (GlcNAc and GlcN) in core demonstrate retention of inner and outer core. Both 1H NMR and 13C NMR experiments were measured in D2O at 25 °C in Shigemi NMR tube, at 600 MHz (150 MHz for 13C NMR) on a Bruker Avance Spectrometer with a TCI cryoprobe. Samples were prepared at ~10 mg/mL concentration.