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. 2023 Sep 15;19(9):e1011182. doi: 10.1371/journal.ppat.1011182

Fig 3. Antibodies to PfRH5 and PfCyRPA modify temporal aspects of the pre- and post-invasion phases of Plasmodium falciparum into human RBCs.

Fig 3

(A-E) The invasion steps being monitored from live cell videos of antibody-treated parasites are indicated on the y-axes. The antibody types and concentrations are indicated on the x-axes. Anti-PfRH5 (orange, pink) and Cy.009 (green) antibodies increase the length of the preinvasion phase from first erythrocyte contact to the start of merozoite penetration. The Cy.003 (dark blue) and Cy.007 IgG and Fabs (purples) tend to increase the length of the merozoite internalization period. (F) Cy.007 antibodies decrease the numbers of echinocytosis events per egress and (G-H) R5.008 (pink) and Cy.009 (green) decrease the time from the end of invasion/deformation to echinocytosis. Full antibody names and concentrations (μg/mL) are indicated below bottom graphs. Antibody 8 indicates Cy.007 Fab-(133). Symbols represent each event, and the median is shown by black bars. Statistical analyses were performed using unpaired t tests in GraphPad Prism V 9.0. The asterisks indicate where parasite mAbs have altered the number of events significantly from the EBL 040 control with *p<0.05, **p<0.01 and ***p<0.001.