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. Author manuscript; available in PMC: 2024 Aug 17.
Published in final edited form as: Cell Chem Biol. 2023 Jun 29;30(8):879–892.e5. doi: 10.1016/j.chembiol.2023.06.007

Figure 7. Selected variants demonstrate altered efficiencies when targeted to other sites.

Figure 7.

The wild type (WT) and ten selected scaffolds were retargeted to five other target sites on the GFP gene, and GFP knockout was assessed in cells. GFP Target 1, the DNA site utilized during selection, is provided for comparison. The degree of GFP knockout is shown for each combination of scaffold variant with each DNA target domain. White colored boxes represent no knockout, black indicates 50% knockout, and red represents >80% reduction in GFP 10 expressing cells (significance values are reported in Data.S2; n= 2–5 independent experiments for each of the 66 GFP targeted sgRNAs in each of the two cell types).